GB/T 23501-2009 English PDF

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GB/T 23501-2009: Determination of T-2 toxin in food -- High performance liquid chromatographic method with immunoaffinity column clean-up
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 23501-2009	239	Add to Cart	3 days	Determination of T-2 toxin in food -- High performance liquid chromatographic method with immunoaffinity column clean-up	Obsolete

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Basic data

Standard ID: GB/T 23501-2009 (GB/T23501-2009)
Description (Translated English): Determination of T-2 toxin in food -- High performance liquid chromatographic method with immunoaffinity column clean-up
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 6,634
Date of Issue: 2009-04-08
Date of Implementation: 2009-05-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the immunoaffinity chromatography and HPLC purification methods T-2 toxin in food content. This standard applies to food and food products, determination of butter and butter products in T-2 toxin content, liquor, sauce, vinegar.

GB/T 23501-2009: Determination of T-2 toxin in food -- High performance liquid chromatographic method with immunoaffinity column clean-up

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of T-2 toxin in food. High performance liquid chromatographic method with immunoaffinity column clean-up ICS 67.050 X04 National Standards of People's Republic of China T-2 toxin DETERMINATION Immunoaffinity chromatography and HPLC purification Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the National Food Industry Standardization Technical Committee (SAC/TC64) proposed. This standard by the National Standardization Technical Committee on Food Universal Detection Technology Food Industry Technical Committee (SAC/TC64/SC8) Centralized. This standard was drafted. Qingdao City Product Quality Supervision and Inspection, People's Republic of China Shandong Exit Inspection and Quarantine, the inspection Wellcome Technology Co., Ltd., Tsingtao Brewery. The main drafters of this standard. Zhanghui Zhen, Li Huiying, Wang Xiaobin, Hao Junguang, Bao Lei, Sun Xiao Jie, Wang Xiong. T-2 toxin DETERMINATION Immunoaffinity chromatography and HPLC purification

1 Scope

This standard specifies the immunoaffinity chromatography and high performance liquid chromatographic method for purifying T-2 toxin in food content. This standard applies to the determination of food products, wine, soy sauce, vinegar, soy sauce and sauce products in T-2 toxin levels and food. The standard method detection limits. Food and food products detection limit of 10μg/kg, alcohol detection limit of 5μg/kg, soy sauce, vinegar, Butter and butter products detection limit of 5μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

Extracting a sample of T-2 toxin with extracts by immunoaffinity column cleanup after 1- nitrile anthracene derivatized by HPLC with fluorescence detection Measuring devices, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water line with GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. Extract 4.3. methanol + water (80 + 20). 4.4 4-dimethylaminopyridine (DMAP). 0.325g/L. Weigh 0.0325g4- dimethylaminopyridine, dissolved and set the volume with toluene To 100mL. 4.5 anthracene carbonitrile 1- (1-anthroylnitrile, 1-AN). 0.3g/L. Weigh 0.030g1- anthracene nitrile, toluene was dissolved and set the volume to 100mL. 4.6 T-2 toxin standard. Purity ≥98%. 4.7 T-2 toxin stock standard solution. Weigh accurately an amount of T-2 toxin standards, dissolved in acetonitrile, dubbed 0.1mg/mL standard Stock solution stored at -20 ℃ in the dark can be used for 3 months. 4.8 T-2 toxin standard working solution. Depending on needs, accurately draw a certain amount of T-2 toxin standard stock solution was diluted with acetonitrile, respectively Dubbed equivalent to 5ng/mL, 10ng/mL, 50ng/mL, 100ng/mL, working standard solution 200ng/mL of, 4 ℃ preservation, can With 7d. 4.9 T-2 toxin immunoaffinity column. 4.10 glass fiber filter. diameter 11cm, pore size 1.5μm, no fluorescent properties. 5. Apparatus 5.1 Balance. a sense of the amount of 0.001g. 5.2 High performance liquid chromatograph. equipped with fluorescence detector. ......

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