GB/T 22976-2008 English PDF

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GB/T 22976-2008: Determination of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder -- LC-MS-MS method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22976-2008	229	Add to Cart	3 days	Determination of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder -- LC-MS-MS method	Valid

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Basic data

Standard ID: GB/T 22976-2008 (GB/T22976-2008)
Description (Translated English): Determination of ��-trenbolone, ��-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder -- LC-MS-MS method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 10,120
Date of Issue: 2008-12-31
Date of Implementation: 2009-05-01
Quoted Standard: GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2008 No.26 (Total No.139)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the milk and milk powder ��- trenbolone, ��- trenbolone, 19-nortestosterone ethylene and ethylene- epi-19- nortestosterone residues performance liquid chromatography-tandem mass spectrometry. This standard applies to milk and milk powder ��- trenbolone, ��- trenbolone, 19-nortestosterone ethylene and ethylene- epi-19- nortestosterone residue determination and confirmation. The detection limit of the standard methods: Milk ��- trenbolone, ��- trenbolone, 19-nortestosterone ethylene and ethylene- epi-19- nortestosterone is 1��g/kg, milk powder ��- trenbolone, ��- trenbolone, 19-nortestosterone ethylene and ethylene- epi-19- nortestosterone is 5��g/kg.

GB/T 22976-2008: Determination of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder -- LC-MS-MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder. LC-MS-MS method ICS 67.050 X04 National Standards of People's Republic of China Milk and milk powder α- trenbolone, β- trenbolone, 19- Determination of residues Liquid chromatography - tandem mass spectrometry Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard is drafted by. People's Republic of China Exit Inspection and Quarantine Qinhuangdao, Hebei People's Republic of China Entry-Exit Inspection Quarantine Bureau. The main drafters of this standard. Yilian Feng, Wang Fengchi, segment, Fan, Guo Chunhai, Mayu Song, Jia Haitao, Liu Sheng, hook Jin, Pang Guofang. Milk and milk powder α- trenbolone, β- trenbolone, 19- Determination of residues Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the milk and milk powder α- trenbolone, β- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues High performance liquid chromatography - tandem mass spectrometry. This standard applies to milk and milk powder α- trenbolone, β- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues Determination and confirmation. The detection limit of the standard methods. milk α- trenbolone, β- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone as 1μg/kg, milk powder α- trenbolone, β- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone was 5μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1- 2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Sample at pH5.0 enzyme hydrolysis conditions, acetonitrile - ethyl acetate extraction, gel chromatography purification by high performance liquid chromatography - tandem mass spectrometry measurement Fixed, external standard.

4 Reagents and materials

Unless otherwise stated, all reagents HPLC grade water to a water GB/T 6682 regulations. 4.1 acetonitrile. 4.2 ethyl acetate. 4.3 cyclohexane. 4.4 methanol. 4.5 acetic acid. 4.6 anhydrous sodium sulfate, AR. After burning 650 ℃ 4h, placed in the dryer set aside. 4.7 sodium acetate, analytical grade. 4.8 β- glucuronidase/aryl sulfatase. β-glucuronidase/arylsulfatase, 100000unit/mL. 4.9 0.02mol/L sodium acetate buffer (pH = 5.0). Weigh 0.82g of anhydrous sodium acetate were dissolved in 500mL of water, adjusted with acetic acid pH = 5.0. ......

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