GB/T 22287-2008 English PDF

US$299.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 22287-2008: Detection of hepatitis A virus in shellfish -- Conventional RT-PCR and real-time fluorescence RT-PCR
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22287-2008	299	Add to Cart	3 days	Detection of hepatitis A virus in shellfish -- Conventional RT-PCR and real-time fluorescence RT-PCR	Valid

Similar standards

GB/T 22460   GB/T 22507   GB/T 22224   GB/T 45556   GB/T 15683   

Basic data

Standard ID: GB/T 22287-2008 (GB/T22287-2008)
Description (Translated English): Detection of hepatitis A virus in shellfish -- Conventional RT-PCR and real-time fluorescence RT-PCR
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.100
Word Count Estimation: 13,113
Date of Issue: 2008-08-12
Date of Implementation: 2008-12-01
Quoted Standard: GB/T 6682; GB 19489; SN/T 1193
Regulation (derived from): National Standard Approval Announcement 2008 No.12 (Total No.125)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the hepatitis A virus in shellfish ordinary fluorescent RT-PCR and RT-PCR detection methods. This standard applies to shellfish detect hepatitis A virus nucleic acid.

GB/T 22287-2008: Detection of hepatitis A virus in shellfish -- Conventional RT-PCR and real-time fluorescence RT-PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of hepatitis A virus in shellfish. Contentional RT-PCR and real-time fluorescence RT-PCR ICS 67.100 X04 National Standards of People's Republic of China Detection of hepatitis A virus in shellfish RT-PCR method and ordinary Real-time fluorescent RT-PCR. Posted 2008-08-12 2008-12-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chan Kwong-chuen, Rao red, para Hong An, Feng Qian, Fu Pu Bo, Zhang Huiyuan, Wang Qi, Zeng Jing, Zhang Rui, Li Jinhua. Detection of hepatitis A virus in shellfish RT-PCR method and ordinary Real-time fluorescent RT-PCR.

1 Scope

This standard specifies the hepatitis A virus in shellfish ordinary fluorescent RT-PCR and RT-PCR detection method. This standard applies to shellfish to detect hepatitis A virus nucleic acid.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) GB 19489 General requirements for laboratory biosafety SN/T 1193 genetic analysis and detection laboratory technical requirements

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 DNA template first high temperature denaturation into single strands, at a suitable temperature and buffer, two primers and the template DNA strand two Some complementary sequence on the occurrence of annealing, followed by catalytic DNA polymerase in four dNTP as substrate, annealing of primers to be extended Stretch, and so forth denaturation, annealing and extension that is located between two primer sequences of the DNA fragment was amplified geometrically. 3.2 RNA in the action of reverse transcriptase, under suitable reaction conditions, is reverse transcribed into cDNA, cDNA as template to PCR. 3.3 Real-time fluorescent RT-PCR method is based on the conventional RT-PCR, to add a specific fluorescent probe. The probe is Period of oligonucleotides, both ends of the report marks a fluorophore and a quencher fluorophore. The probe is intact, the fluorescence emitted by the reporter group The optical signal is quencher absorption; PCR amplification, T Rao q enzyme 5'-3 'exonuclease activity of the enzyme degradation of the probe, and to make the report fluorophore Separating quencher fluorophore, fluorescence monitoring system so that the fluorescent signal may be received, i.e., each strand of DNA amplification, there is a Fluorescence Sub formed to achieve a cumulative fluorescent signal PCR product formed completely synchronized. 3.4 When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced.

4 Abbreviations

The following abbreviations apply to this standard. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 22287-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 22287-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 22287-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 22287-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.