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GB/T 22259-2025 English PDF

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US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 22259-2025: Determination of oxytetracycline in feeds
Status: Valid

GB/T 22259: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22259-2025	279	Add to Cart	3 days	Determination of oxytetracycline in feeds	Valid
GB/T 22259-2008	139	Add to Cart	3 days	Determination of oxytetracycline in feeds -- High performance liquid chromatography	Valid

Similar standards

GB/T 51033 GB/T 50082 GB/T 22259

Basic data

Standard ID: GB/T 22259-2025 (GB/T22259-2025)
Description (Translated English): Determination of oxytetracycline in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 14,176
Date of Issue: 2025-08-01
Date of Implementation: 2026-02-01
Older Standard (superseded by this standard): GB/T 22259-2008
Issuing agency(ies): State Administration for Market Regulation, Standardization Administration of China

GB/T 22259-2025: Determination of oxytetracycline in feeds

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT22259-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 22259-2008 Determination of oxytetracycline in feed Released on August 1, 2025 Implementation on February 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 22259-2008 "Determination of oxytetracycline in feed - High performance liquid chromatography method" and is incompatible with GB/T 22259-2008. In addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application, detection limit and quantification limit have been changed (see Chapter 1, Chapter 1 of the.2008 edition); b) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 4); c) The principle has been changed (see 5.1, Chapter 3 of the.2008 edition); d) The reagents or materials have been changed (see 5.2, Chapter 4 of the.2008 edition); e) The instrumentation has been changed (see 5.3, Chapter 5 of the.2008 edition); f) The sample has been changed (see 5.4, Chapter 6 of the.2008 edition); g) The test steps have been changed (see 5.5, Chapter 7 of the.2008 edition); h) The test data processing has been changed (see 5.6, Chapter 8 of the.2008 edition). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Henan Agricultural, Animal and Aquatic Products Inspection Technology Research Institute and Shaanxi Animal Husbandry Technology Extension Center. The main drafters of this document are. Wu Ningpeng, Peng Li, Li Hong, Li Huisu, Cui Chao, Hu Yanli, Zhang Zhenyu, Zhang Chongwei, Chao Juanjuan, Meng Lei, and Jia Yuhua. The previous versions of this document and the documents it replaces are as follows. ---First published in.2008 as GB/T 22259-2008; ---This is the first revision. Determination of oxytetracycline in feed

1 Scope

This document describes a method for the determination of oxytetracycline in feed by liquid chromatography-tandem mass spectrometry and high performance liquid chromatography. This document applies to compound feeds, concentrated feeds, concentrate supplements, additive premixes, feed ingredients and mixed feed additives Determination of oxytetracycline in. The detection limit of this document liquid chromatography-tandem mass spectrometry is 0.02mg/kg and the quantification limit is 0.05mg/kg; high performance liquid chromatography combined with The detection limit for feed (except aquatic feed), concentrated feed, concentrate supplement and feed raw materials is 1.0 mg/kg and the quantification limit is 2.0 mg/kg. The detection limit of aquatic feed is 5.0 mg/kg and the quantification limit is 10.0 mg/kg. The limits of detection of premixed feed and mixed feed additives are 5.0 mg/kg and 10.0 mg/kg respectively. The detection limit is 2.5 mg/kg and the quantification limit is 5.0 mg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document. 4 Liquid chromatography-tandem mass spectrometry (LC-MS/MS) 4.1 Principle Oxytetracycline in the sample was extracted with hydrochloric acid methanol solution or buffer solution, the extraction solution was diluted or purified by HLB solid phase extraction column, and the liquid phase was used to extract the oxytetracycline. The product was determined by chromatography-tandem mass spectrometry, calibrated by matrix-matched standard curve, and quantified by external standard method. 4.2 Reagents or materials Unless otherwise specified, only analytical grade reagents were used. 4.2.1 Water. GB/T 6682, Grade 1. 4.2.2 Acetonitrile. chromatographic grade. 4.2.3 Methanol. chromatographic grade. 4.2.4 Formic acid. chromatographically pure. 4.2.5 Hydrochloric acid methanol solution. Pipette 20 mL of hydrochloric acid and dissolve it in 1000 mL of methanol and mix well. 4.2.6 Hydrochloric acid solution (1 mol/L). Pipette 9 mL of hydrochloric acid, dilute to 100 mL with water, and mix thoroughly. 4.2.7 Sodium hydroxide solution (1 mol/L). Weigh 4 g of sodium hydroxide, dissolve it in water and dilute to 100 mL, then mix thoroughly. 4.2.8 Buffer Solution I. Weigh 12.9 g of citric acid monohydrate, 27.6 g of disodium hydrogen phosphate dodecahydrate, and 27.6 g of disodium ethylenediaminetetraacetic acid dihydrate. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 22259-2025_English be delivered?

Answer: Upon your order, we will start to translate GB/T 22259-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 22259-2025_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 22259-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 22259-2025?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 22259-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.