GB/T 21769-2008 English PDF

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GB/T 21769-2008: Chemical -- In vitro 3T3 NRU phototoxicity test method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21769-2008	314	Add to Cart	3 days	Chemical -- In vitro 3T3 NRU phototoxicity test method	Valid

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Basic data

Standard ID: GB/T 21769-2008 (GB/T21769-2008)
Description (Translated English): Chemical -- In vitro 3T3 NRU phototoxicity test method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 11.100
Word Count Estimation: 16,197
Date of Issue: 2008-05-12
Date of Implementation: 2008-09-01
Quoted Standard: EU 67/548/EEC
Adopted Standard: OECD No.432-2004, IDT
Regulation (derived from): Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the chemicals in vitro 3T3 neutral red uptake phototoxicity test range, normative references, terms and definitions, abbreviations, testing basic principles, test methods, test results and test reports. This standard applies to chemicals of potential phototoxicity screening and detection. This standard can be used as animal testing of chemicals phototoxicity alternative methods.

GB/T 21769-2008: Chemical -- In vitro 3T3 NRU phototoxicity test method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemical. In vitro 3T3 NRU phototoxicity test method ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemicals in vitro 3T3 neutral red uptake Phototoxicity test methods Posted 2008-05-12 2008-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is identical with the Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No. 432 (2004) "In vitro 3T3 Neutral Red uptake phototoxicity test "(in English). The editorial changes made the following standard. --- Increasing the scope and normative references partial section; --- Introduction to the OECD test methods portion and principles of consolidation; --- Know Before test and test methods OECD merger; --- Deleted the reference section of the OECD; --- Evaluation of the test results with reference to the test methods of the EU. Appendix A of this standard and Appendix B is an informative annex. This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points. This standard is drafted by. Guangdong CIQ. The main drafters of this standard. Cheng Shujun, Xuchong Hui, Jiao Hong, Wenqiao Ling, PAN Fang, Chen Qiang, Qin Yao, Li Qingxiang. Chemicals in vitro 3T3 neutral red uptake Phototoxicity test methods

1 Scope

This standard specifies the chemical in vitro 3T3 neutral red uptake phototoxicity test scope, normative references, terms and definitions, abbreviations Language, the basic principles of the test, test methods, test results and test report. This standard applies to screening and detection of chemical potential phototoxicity. This standard can phototoxicity animal testing as an alternative to chemicals one.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No. 101 (1981), "Chemicals UV-visible absorption spectroscopy" (English Chinese Version). EU directive EU67/548/EEC Annex VB. 41 (2000) "in vitro neutral red uptake phototoxicity phototoxicity test" (English Chinese Version). 3 Terms and definitions, abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this standard. 3.1.1 Incident to ultraviolet (UV) or visible light of a surface (Vis) intensity in watts (W/m2) or milliwatts per square meter per square Centimeter (mW/cm2). 3.1.2 Is incident to a surface of the ultraviolet or visible light quantity (intensity × time), in joules per square meter (J/m2) or joules per square Cm (J/cm2). 3.1.3 International Commission on Illumination (CIE, CommissionInternationaledeL'Eclairage) recommended is defined as. UVA (315nm ~ 400nm), UVB (280nm ~ 315nm) and UVC (100nm ~ 280nm). Some other definitions may be used; UVB and UVA boundaries typically 320nm, UVA can dispose of at 340nm UV-A1 and UV-A2. 3.1.4 Measuring total activity of a cell population parameters (such as lysosomal neutral red dye uptake activity), its value depends on the end point measured and Experimental design used, and related to the total number of cells and/or cell viability. ......

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