GB/T 21498-2008 English PDF

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GB/T 21498-2008: Determination of trypsin inhibitor activity of soya products
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21498-2008	479	Add to Cart	3 days	Determination of trypsin inhibitor activity of soya products	Obsolete

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Basic data

Standard ID: GB/T 21498-2008 (GB/T21498-2008)
Description (Translated English): Determination of trypsin inhibitor activity of soya products
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X14
Classification of International Standard: 67.200.20
Word Count Estimation: 12,153
Date of Issue: 2008-03-06
Date of Implementation: 2008-08-01
Quoted Standard: GB/T 6682
Adopted Standard: ISO 14902-2001, IDT
Regulation (derived from): State Health and Family Planning Commission Notice No. 11 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the determination of soybean trypsin inhibitor activity. Trypsin inhibitor activity can be used to indicate the degree of roast soy products. This standard applies to the determination of soybean trypsin inhibitor activity.

GB/T 21498-2008: Determination of trypsin inhibitor activity of soya products

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of trypsin inhibitor activity of soya products ICS 67.200.20 X14 National Standards of People's Republic of China GB/T 21498-2008/ISO 14902.2001 Soybean trypsin inhibitor Determination of Activity (ISO 14902.2001, IDT) Posted 2008-03-06 2008-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is identical with ISO 14902.2001 "animal feed soybean trypsin inhibitor activity assay" (in English). For ease of use, the standard ISO 14902.2001 includes the following editorial changes. --- The "this International Standard" by "this standard"; --- Decimal by decimal points instead of the original text, "", "."; --- Deleting the original standard name of "animal feed" the term used directly "soybean"; --- The Normative references in the "ISO 3696. 1987 for analytical laboratory use specifications and test methods" was changed to "Laboratory use - Specification and test methods GB/T 6682 analysis"; --- On the forms increases the header; --- The relevant formulas are numbered. Appendix A of this standard is a normative appendix, Appendix B is an informative annex. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard is drafted by. Nanjing University of Finance and Economics. The main drafters of this standard. OILS, Yuan Jian, Wang Haifeng, Yang Xiaorong. GB/T 21498-2008/ISO 14902.2001 Soybean trypsin inhibitor Determination of Activity

1 Scope

This standard specifies the method for determination of soybean trypsin inhibitor activity. Trypsin inhibitor activity may be used to represent a large Browning soy. This standard applies to the determination of soybean trypsin inhibitor activity. The method detection limit was 0.5mg/g.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 According to the procedures specified in the standard measure of the quality of trypsin inhibitor divided by the sample mass obtained. NOTE. trypsin inhibitor activity in mg/g expressed. Principle 4 Under pH9.5 conditions, trypsin inhibitor extracted from the sample. By addition of the substrate benzoyl -L- arginine - p-nitroanilide (L-BAPA) measuring residual trypsin activity. The amount determined by spectrophotometry release of p-nitroaniline.

5 Reagents and materials

Use only analytical reagent. 5.1 Water. the lowest level 3, in line with GB/T 6682 requirements. 5.2 Sodium hydroxide solution. Ba (NaOH) = 0.01mol/L. 5.3 hydrochloric acid solution. Ba (HCl) = 6mol/L. 5.4 hydrochloric acid solution. Ba (HCl) = 1mol/L. 5.5 hydrochloric acid solution. Ba (HCl) = 0.1mol/L. 5.6 hydrochloric acid solution. Ba (HCl) = 0.001mol/L. Acetic acid solution 5.7. Ba (CH3COOH) = 5.3mol/L. 5.8 calcium chloride (CaCl2 · 2H2O). 5.9 calcium chloride hydrochloric acid solution. Weigh 0.735g calcium chloride (5.8) was dissolved in 1L0.001mol/L hydrochloric acid solution (5.6), the pH is adjusted To 3.0 ± 0.1. GB/T 21498-2008/ISO 14902.2001 ......

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