GB/T 15400-2018 English PDF

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GB/T 15400-2018: Determination of tryptophan in feeds
Status: Valid

GB/T 15400: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 15400-2018	199	Add to Cart	3 days	Determination of tryptophan in feeds	Valid
GB/T 15400-1994	199	Add to Cart	2 days	Determination of tryptophan in feedstuffs. Spectrophotometry	Obsolete

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Basic data

Standard ID: GB/T 15400-2018 (GB/T15400-2018)
Description (Translated English): Determination of tryptophan in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 10,193
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Older Standard (superseded by this standard): GB/T 15400-1994
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 15400-2018: Determination of tryptophan in feeds

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of tryptophan in feeds ICS 65.120 B46 National Standards of People's Republic of China Replace GB/T 15400-1994 Determination of tryptophan in feed Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 15400-1994 "Determination of tryptophan in feed by spectrophotometry". Compared with GB/T 15400-1994, this standard changes the main technical contents except for editorial changes. --- Added high performance liquid chromatography, modified the applicable object, and increased the limit of quantitation of the two methods (see Chapter 1,.1994 edition) Chapter 1). --- Revised the expression of the principle of spectrophotometry (see Chapter 4.1, Chapter 3 of the.1994 edition). --- Revised the expression of precision (see 4.7, 8.3 of the.1994 edition). This standard is proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76). This standard was drafted. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center (Beijing)], Tongwei Co., Ltd. The main drafters of this standard. Li Lan, Jia Wei, Zhao Yan, Zhao Genlong, Song Jun, Zhang Fengqi. The previous versions of this standard were released as follows. ---GB/T 15400-1994. Determination of tryptophan in feed

1 Scope

This standard specifies high performance liquid chromatography and spectrophotometry for the determination of tryptophan in feed. This standard applies to the determination of tryptophan in feed ingredients, compound feed, concentrated feed and concentrate supplements. The limit of quantitation of high performance liquid chromatography is 0.02%; when the maximum sample weight of the sample is 700mg, and the dilution factor of the hydrolyzate is 25 times, The limit of quantitation of the photometric method is 0.04%.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6433 Determination of crude fat in feed GB/T 6682 Analytical laboratory water specifications and test methods GB/T 14699.1 Feed sampling Preparation of GB/T 20195 animal feed samples 3 high performance liquid chromatography (arbitration method) 3.1 Principle The tryptophan in the sample is hydrolyzed by lithium hydroxide solution, separated by high performance liquid chromatography, detected by ultraviolet or fluorescence detector, external standard method Quantitative. 3.2 Reagents or materials Unless otherwise specified, the reagents used in this standard are of analytical grade. 3.2.1 Water. Meet the requirements of the first grade water in GB/T 6682. 3.2.2 Methanol. chromatographically pure. 3.2.3 Potassium hydroxide solution (0.1mol/L). Weigh 0.56g of potassium hydroxide and dissolve it in 100mL of water. 3.2.4 Lithium hydroxide solution (4mol/L). Weigh 83.9g of lithium hydroxide and dissolve it in 500mL of water. 3.2.5 sodium acetate buffer solution (0.0085mol/LNa, pH=4.5). Weigh 0.70g of anhydrous sodium acetate and dissolve it in 1000mL of water. The pH was adjusted to 4.5 with glacial acetic acid. 3.2.6 Mobile phase. sodium acetate buffer solution (3.2.5) methanol (3.2.2) = 95 5. 3.2.7 L-tryptophan standard stock solution. accurately weigh 25mg (accurate to 0.0001g) tryptophan reference substance (purity greater than 99%), set In a 25 mL beaker, add potassium hydroxide solution (3.2.3) dropwise with a dropper to dissolve it, and quantitatively transfer it to a 250 mL brown volumetric flask. The volume was adjusted to a concentration of 100 μg/mL. Store at 2 ° C ~ 8 ° C, valid for 1 month. 3.2.8 L-tryptophan standard series solution. accurately remove L-tryptophan standard stock solution (3.2.7) 5.00mL, 7.50mL, 10.00 mL, 12.50 mL, 15.00 mL, and 17.50 mL were placed in a 25 mL brown volumetric flask, and the volume was adjusted to the mark with water and shaken. its The solution concentrations were 20 μg/mL, 30 μg/mL, 40 μg/mL, 50 μg/mL, 60 μg/mL, and 70 μg/mL, respectively. 2 ° C ~ 8 ° C Save, valid for 2 weeks. ......

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