GB/T 14489.2-2008 English PDF

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GB/T 14489.2-2008: Inspection of grain and oils -- Determination of crude protein in oilseeds
Status: Obsolete

GB/T 14489.2: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 14489.2-2008	279	Add to Cart	3 days	Inspection of grain and oils -- Determination of crude protein in oilseeds	Obsolete
GB/T 14489.2-1993	199	Add to Cart	2 days	Method for determination of crude protein oilseed	Obsolete

Similar standards

GB/T 11764   GB/T 15681   GB/T 11762   GB/T 11761   GB/T 14489.1   

Basic data

Standard ID: GB/T 14489.2-2008 (GB/T14489.2-2008)
Description (Translated English): Inspection of grain and oils -- Determination of crude protein in oilseeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B33
Classification of International Standard: 67.200
Word Count Estimation: 7,790
Date of Issue: 2008-11-04
Date of Implementation: 2009-01-20
Older Standard (superseded by this standard): GB/T 14489.2-1993
Regulation (derived from): National Standard Approval Announcement 2008 No.18 (Total No.131)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the terms and definitions of plant oil crude protein determination, principle, reagents, instrument, sample preparation, operating procedures and the results of calculations. This standard applies to the determination of crude protein in plant oil.

GB/T 14489.2-2008: Inspection of grain and oils -- Determination of crude protein in oilseeds

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils. Determination of crude protein in oilseeds ICS 67.200 B33 National Standards of People's Republic of China Replacing GB/T 14489.2-1993 Inspection of grain and oil plants Determination of crude protein Posted 2008-11-04 2009-01-20 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is GB/T 14489.2-1993 "oil crude protein assay" amendments. This standard and GB/T 14489.2-1993 main technical differences are as follows. --- Modify the normative references; --- To increase and modify the terms and definitions; --- Revised reagents and equipment instructions; --- Revised sample preparation; --- Increasing the semi-micro method and instrument method; --- Increased inspection distillation step; --- Revised calculation results. From the date of implementation of this standard instead of GB/T 14489.2-1993. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. State Grain Reserve Wuxi Scientific Research and Design Institute, East China Sea Oil Industries (Zhangjiagang) Co., Ltd. The main drafters of this standard. Qin Weiguo, Zhang Chunhui, Chuli Xia, Wang Lan, Wang Yuming, Zhou Renkai. This standard replaces the standards previously issued as follows. --- GB/T 14489.2-1993. Inspection of grain and oil plants Determination of crude protein

1 Scope

This standard specifies the terms and definitions of crude oil plant protein assay, principle, reagents, apparatus, sample preparation, procedures, and results Calculations. This standard applies to the determination of crude oil plant protein.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Preparation of GB/T 601 chemical reagent standard titration solution GB 5491 grain and oilseeds testing for sampling, and sample method

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Under the conditions specified in this standard to determine the content of vegetable oil feed nitrogen conversion to give the crude protein content. Principle 4 In the presence of a catalyst, sulfuric acid digestion of organic matter in the sample, so that nitrogen was converted into ammonium sulfate. Adding alkali and distillation of ammonia slip Out with boric acid to absorb ammonia, nitrogen content calculated using standard acid titration, multiplied by the appropriate conversion factor to calculate the protein content of crude protein.

5 Reagents

All reagents should be nitrogen-free compounds, which were of analytical grade purity, the water used should be distilled water. 5.1 sulfate. 18mol/L. 5.2 mixed catalyst. 0.4g copper sulfate (CuSO4 · 5H2O), 6g potassium or sodium sulfate, milled mix. 5.3 Sodium hydroxide solution. Ba (NaOH) = 400g/L, 400g of sodium hydroxide dissolved in water and dilute to 1000mL. 5.4 boric acid solution. Ba (H3BO3) = 20g/L, 20g of boric acid dissolved in water and dilute to 1000mL. Mixed indicator 5.5. 1 parts by 1g/L methyl red in ethanol (95%) and 5 parts of a solution of 1g/L of bromocresol green in ethanol (95%) was uniformly mixed. 5.6 hydrochloric acid standard solution. Ba (HCl) = 0.1mol/L, hydrochloric acid standard solution preparation and calibration according to GB/T 601 executed. 5.7 sucrose. 5.8 Ammonium sulfate. dry. 5.9 boric acid absorption solution. 10g/L boric acid solution 1000mL, was added 1g/L of bromocresol green in ethanol (95%) solution of 10mL, 1g/L methyl Red ethanol solution 7mL, 400g/L sodium hydroxide solution 0.5mL, mixing, cool place, storage period of one month (with automatic program). 5.10 Methyl red indicator. 0.1g methyl red dissolved in 100mL of ethanol (95%).

6 Instruments

Commonly used laboratory equipment and the following special equipment. ......

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