GB 5009.6-2025 English PDFUS$319.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 5009.6-2025: National food safety standard - Determination of fat in foods Status: Valid GB 5009.6: Historical versions
Basic dataStandard ID: GB 5009.6-2025 (GB5009.6-2025)Description (Translated English): National food safety standard - Determination of fat in foods Sector / Industry: National Standard Classification of Chinese Standard: C53 Word Count Estimation: 16,131 Date of Issue: 2025-09-02 Issuing agency(ies): National Health Commission; State Administration for Market Regulation GB 5009.6-2025: National food safety standard - Determination of fat in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.6-2025National Standards of the People's Republic of China National Food Safety Standards Determination of fat in food Published on 2025-09-02 Implemented on 2026-03-02 National Health Commission of the People's Republic of China State Administration for Market Regulation issued ForewordThis standard replaces GB 5009.6-2016 "National Food Safety Standard - Determination of Fat in Food". Compared with GB 5009.6-2016, the main changes in this standard are as follows. ---The applicability of the first method (Soxhlet extraction), the second method (acid hydrolysis), the third method (alkaline hydrolysis), and the fourth method (Geb process) has been modified. scope; ---Added sample preparation methods for the first method (Soxhlet extraction), the second method (acid hydrolysis), and the third method (alkali hydrolysis); ---Specifies the precision of oil samples in the first Soxhlet extraction method; ---The extraction solvent for the second acid hydrolysis method has been modified; ---Increased the volume fraction of sulfuric acid in the fourth Geb process. National Food Safety Standards Determination of fat in food 1.Scope This standard specifies the method for determining the fat content in food. The first method of this standard, Soxhlet extraction, is applicable to the determination of free fats in food. The second method of this standard, acid hydrolysis, is applicable to the determination of fat in food (excluding milk and dairy products). The third method of this standard, alkaline hydrolysis, is applicable to the determination of fat in milk and dairy products, special dietary foods, and protein beverages. The fourth method of this standard, the Geb method, is applicable to the determination of fat in raw milk, sterilized milk, and pasteurized milk. First method of Soxhlet extraction 2.Principle The sample was extracted directly with solvents such as anhydrous diethyl ether or petroleum ether, the solvent was evaporated to remove the solvent, and then dried to obtain the content of free fat. 3.Reagents and Materials Unless otherwise stated, all reagents used in this method are of analytical grade, and the water is grade III water as specified in GB/T 6682. 3.1 Reagents 3.1.1 Anhydrous diethyl ether (C4H10O). 3.1.2 Petroleum ether (CnH2n 2). The boiling range of petroleum ether is 30℃~60℃. 3.2 Materials 3.2.1 Quartz sand. 3.2.2 Absorbent cotton. 4.Instruments and Equipment 4.1 Homogenizer. 4.2 Tissue grinder or pulverizer. 4.3 Soxhlet extractor. 4.4 Constant temperature water bath. 4.5 Analytical balance. sensitivity of 0.001g and 0.0001g. 4.6 Electric heating forced-air drying oven. 4.7 Dryer. Contains an effective desiccant, such as silica gel. 4.8 Filter paper tube. 4.9 Evaporating dish. 5.Analysis Steps 5.1 Sample Preparation For particle-free and homogeneous liquid samples, shake well directly before use. For liquid samples with particles, non-homogeneous liquid samples, or semi-solid samples, use a homogenizer to mix them before use. For solid samples, use a tissue homogenizer or grinder to pulverize and mix thoroughly before use. For frozen drinks, heat appropriately to melt and stir thoroughly while hot. Mix thoroughly and set aside. The prepared sample should be tested as soon as possible. Note. Oil samples need to be dried at 105℃±2℃ for 1 hour, then crushed and passed through a sieve with a pore size of 0.425mm. 5.2 Sample Preparation 5.2.1 Solid sample. Weigh 2g to 5g of uniform sample (accurate to 0.001g) and transfer it all into the filter paper tube. 5.2.2 Liquid or semi-solid samples. Weigh 5g to 10g of a homogeneous sample (accurate to 0.001g), place it in an evaporating dish, and add approximately 20g of quartz. The sand was evaporated to dryness in a boiling water bath, dried in an electric hot air drying oven at 100℃±5℃ for 30 minutes, removed, ground into a fine powder, and then transferred entirely into a filter paper tube. The evaporating dish and the glass rod with the sample attached were wiped clean with degreased cotton soaked in ether, and the cotton was placed in the filter paper tube. 5.3 Extraction Place the filter paper tube into the extraction chamber of the Soxhlet extractor, connect it to a receiving flask that has been dried to constant weight (accurate to 0.0001 g), and extract from the extractor. Add anhydrous diethyl ether or petroleum ether to the upper end of the condenser until it reaches two-thirds of the bottle's volume. Heat the bottle in a water bath at 50°C to 60°C until the anhydrous diethyl ether... Alternatively, extract using petroleum ether with continuous reflux (6-8 times/hour), typically for 6-10 hours. At the end of extraction, collect 1 drop using a ground glass rod. Extraction is complete when no oil spots are found on the ground glass rod. 5.4 Weighing Remove the receiving bottle, recover anhydrous diethyl ether or petroleum ether, and when 1-2 mL of solvent remains in the receiving bottle, evaporate it to dryness in a 60°C water bath. Dry at 100℃±5℃ for 1 hour, then cool to room temperature in a desiccator and weigh (accurate to 0.0001g). Repeat the above steps until constant weight is achieved. (Until the difference between the two weighings does not exceed 2mg), take the result of the smallest weighing. 6.Presentation of Analysis Results The fat content in the sample is calculated according to formula (1). X= m1-m0 m2 × 100 (1) In the formula. X --- The fat content in the sample, expressed in grams per 100 grams (g/100g); m1 --- The content of fat in the receiving flask after constant weight, in grams (g); m0 --- The mass of the receiving bottle, in grams (g); m2 --- Mass of the sample, in grams (g); 100---Conversion factor. The calculation result is expressed to two decimal places. Note. The formula for calculating the fat content in oilseeds shall be in accordance with the formula specified in the relevant standards.7 PrecisionThe absolute difference between two independent measurements obtained under repeatability conditions must not exceed 10% of the arithmetic mean for oil samples. Similarly, it should not exceed 1%. Second method. acid hydrolysis8 PrinciplesBound fats in food are released using strong acids; the released fats are readily soluble in organic solvents. The sample is then hydrolyzed with hydrochloric acid and then... Extracted with anhydrous diethyl ether and petroleum ether, the total content of free and bound fats is obtained by removing the solvent. 9.Reagents and Materials Unless otherwise stated, all reagents used in this method are of analytical grade, and the water is grade III water as specified in GB/T 6682. 9.1 Reagents 9.1.1 Hydrochloric acid (HCl). 9.1.2 Ethanol (C2H5OH). volume fraction of at least 95%. 9.1.3 Anhydrous diethyl ether (C4H10O). 9.1.4 Petroleum ether (CnH2n 2). The boiling range of petroleum ether is 30℃~60℃. 9.1.5 Iodine (I2). 9.1.6 Potassium iodide (KI). 9.2 Reagent Preparation 9.2.1 Hydrochloric acid solution (2 mol/L). Measure 50 mL of hydrochloric acid, add it to 250 mL of water, and mix well. 9.2.2 Iodine solution (0.05mol/L). Weigh 6.5g of iodine and 25g of potassium iodide, dissolve them in a small amount of water, and dilute to 1L. 9.2.3 Diethyl ether-petroleum ether mixture (1+1). Take equal volumes of anhydrous diethyl ether and petroleum ether, mix well and set aside. 9.3 Materials 9.3.1 Blue litmus paper. 9.3.2 Absorbent cotton. 9.3.3 Filter paper. medium speed. 10.Instruments and Equipment 10.1 Homogenizer. 10.2 Tissue grinder or pulverizer. 10.3 Constant temperature water bath. 10.4 Heating plate. meets the high temperature requirement of.200℃. 10.5 Conical flask. 10.6 Analytical balance. sensitivity of 0.1g and 0.001g. 10.7 Electric heating forced-air drying oven. 10.8 Centrifuge. 11.Analysis Steps 11.1 Sample Preparation For particle-free and homogeneous liquid samples, shake well directly before use. For liquid samples with particles, non-homogeneous liquid samples, or semi-solid samples, use a homogenizer to mix them before use. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 5009.6-2025_English be delivered?Answer: Upon your order, we will start to translate GB 5009.6-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 5009.6-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.6-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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