GB 5009.204-2025 English PDFUS$319.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB 5009.204-2025: National food safety standard - Determination of acrylamide in food Status: Valid GB 5009.204: Historical versions
Basic dataStandard ID: GB 5009.204-2025 (GB5009.204-2025)Description (Translated English): National food safety standard - Determination of acrylamide in food Sector / Industry: National Standard Classification of Chinese Standard: X04 Word Count Estimation: 16,166 Date of Issue: 2025-09-02 Issuing agency(ies): National Health Commission; State Administration for Market Regulation GB 5009.204-2025: National food safety standard - Determination of acrylamide in food---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.204-2025National Standards of the People's Republic of China National Food Safety Standards Determination of Acrylamide in Food Published on 2025-09-02 Implemented on 2026-03-02 National Health Commission of the People's Republic of China State Administration for Market Regulation issued ForewordThis standard replaces GB 5009.204-2014 "National Food Safety Standard - Determination of Acrylamide in Food". Compared with GB 5009.204-2014, the main changes in this standard are as follows. ---The scope of application of the standard has been modified; ---The first method adds a sample purification method using "matrix dispersion solid-phase extraction"; ---The section on "Ion Trap Tandem Mass Spectrometer" in the first method has been deleted. National Food Safety Standards Determination of Acrylamide in Food 1.Scope This standard specifies the method for determining acrylamide in food. The first method of this standard, liquid chromatography-mass spectrometry/mass spectrometry, is applicable to the determination of acrylamide in food. The second method of this standard, gas chromatography-mass spectrometry, is applicable to substances other than coffee, tea, sugar, infant formula, and complementary foods for infants. Determination of acrylamide in food. First method. Liquid chromatography-mass spectrometry/mass spectrometry 2.Principle Acrylamide in the sample was extracted with water, purified by matrix dispersion solid-phase extraction, solid-phase extraction column method, or chromatography column method, and then expressed as liquid phase. Chromatography-mass spectrometry/mass spectrometry detection, internal standard method for quantification. 3.Reagents and Materials Unless otherwise stated, all reagents used in this method are of analytical grade, and the water is Grade I water as specified in GB/T 6682. 3.1 Reagents 3.1.1 Formic acid (HCOOH). chromatographic grade. 3.1.2 Methanol (CH3OH). chromatographic grade. 3.1.3 Acetonitrile (CH3CN). chromatographic grade. 3.1.4 n-Hexane (n-C6H14). 3.1.5 Ethyl acetate (CH3COOC2H5). 3.1.6 Anhydrous sodium sulfate (Na2SO4). 3.1.7 Ammonium sulfate [(NH4)2SO4]. 3.1.8 Sodium chloride (NaCl). 3.1.9 Dry ice (CO2). 3.2 Reagent Preparation 3.2.1 Saturated ammonium sulfate solution. Weigh 80g of ammonium sulfate crystals, add 100mL of water, sonicate to dissolve, and let stand at room temperature. Prepare fresh before use. 3.2.2 Formic acid-water solution (0.1%). Accurately transfer 1 mL of formic acid, dilute with water to 1000 mL, and mix thoroughly. Prepare fresh before use. 3.3 Standard Products 3.3.1 Acrylamide (C3H5NO, CAS No.. 79-06-1) standard. purity ≥ 99%, or certified by the national standard substance certificate. Standard product. 3.3.2 13C3-Acrylamide (13C3H5NO, CAS No.. 287399-26-2) Standard. Purity ≥ 98%. 3.4 Preparation of Standard Solutions 3.4.1 Preparation of Acrylamide Standard Solution 3.4.1.1 Acrylamide Standard Stock Solution (1000 mg/L). Accurately weigh 10.0 mg of acrylamide standard (accurate to 0.01 mg), and use... Dissolve and dilute to 10 mL in methanol, mix well, and bring the acrylamide concentration to 1000 mg/L. Transfer the solution to a brown glass container and seal tightly. Store in a sealed container at -18°C, protected from light. Shelf life is 6 months. 3.4.1.2 Acrylamide intermediate solution (100 mg/L). Transfer 1 mL of acrylamide standard stock solution (1000 mg/L) into a 10 mL volumetric flask. Dilute with methanol and bring to volume to the mark, mix well, and the acrylamide concentration should be 100 mg/L. Transfer the solution to a brown glass stopper. Store in a sealed container at -18°C, protected from light. Shelf life is 3 months. 3.4.1.3 Acrylamide working solution I (10 mg/L). Transfer 1 mL of acrylamide intermediate solution (100 mg/L) to a 10 mL volumetric flask, and use... Dilute with water and bring to the mark, mix well to achieve an acrylamide concentration of 10 mg/L. Prepare fresh before use. 3.4.1.4 Acrylamide working solution II (1 mg/L). Transfer 1 mL of acrylamide working solution I (10 mg/L) to a 10 mL volumetric flask, and use... Dilute with water and bring to the mark, mix well, and prepare the acrylamide solution to a concentration of 1 mg/L. Prepare immediately before use. 3.4.2 Preparation of 13C3-acrylamide internal standard solution 3.4.2.1 13C3-Acrylamide Stock Solution (1000 mg/L). Accurately weigh 10.0 mg of 13C3-acrylamide standard (accurate to 0.01 mg). Dissolve and dilute to 10 mL with methanol, mix well, and bring the concentration of 13C3-acrylamide to 1000 mg/L. Transfer the solution to a brown glass container. Store in a sealed container at -18°C, protected from light. Shelf life is 6 months. 3.4.2.2 13C3-Acrylamide Working Solution (10 mg/L). Transfer 1 mL of 13C3-Acrylamide internal standard stock solution (1000 mg/L) to a 100 mL... Dilute with water in a volumetric flask and bring to the mark, mix well, so that the concentration of the 13C3-acrylamide internal standard is 10 mg/L. Prepare fresh before use. 3.4.3 Standard Series Working Fluids Transfer 0.10 mL, 0.50 mL, and 1.00 mL of acrylamide working solution II (1 mg/L) and acrylamide working solution I (10 mg/L) to each solution. Add 0.20 mL, 0.50 mL, and 1.00 mL to a 10 mL volumetric flask, then add 0.10 mL of 13C3-acrylamide internal standard working solution (10 mg/L). Dilute with water to the mark and mix well. The mass concentrations of acrylamide in the standard series solutions were 10 μg/L, 50 μg/L, 100 μg/L, and so on. The internal standard concentration of 13C3-acrylamide is 100 μg/L, with concentrations of.200 μg/L, 500 μg/L, and 1000 μg/L. This can be adjusted based on the actual concentration of the sample solution. Adjust the concentration of the standard series working solutions as needed. Prepare fresh solutions immediately before use. 3.5 Materials 3.5.1 Granular diatomaceous earth. Extralut™ 20 or equivalent. 3.5.2 Matrix dispersion purification tube. containing 20 mg graphitized carbon black, 150 mg octadecyl adsorbent, 150 mg ethylenediamine-N-propylsilane, 200 mg of strong cation exchange material and 150 mg of magnesium sulfate, or equivalent products. 3.5.3 Hydrophilic-lipophilic balanced solid-phase extraction column. The packing material is a copolymer of divinylbenzene and N-vinylpyrrolidone, with a column capacity of 6 mL. The dosage is.200mg, or an equivalent product. 3.5.4 Hybrid silica gel bonded solid-phase extraction column. The packing material is a mixed bond of strong cation exchange and strong anion exchange, with a column capacity of 3 mL. The dosage is.200mg, or equivalent product. 3.5.5 Glass chromatography column. 30cm in length and 1.8cm in inner diameter. 3.5.6 Aqueous microporous filter membranes. 0.22μm and 0.45μm. 4.Instruments and Equipment 4.1 Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), with electrospray ionization source. 4.2 Tissue grinder. 4.3 Rotary evaporator. 4.4 Nitrogen blowing device. 4.5 Oscillator. 4.6 Vortex mixer. 4.7 Electronic balance. sensitivity of 0.01 mg and 1 mg. 4.8 Centrifuge. speed ≥ 10000 r/min. 5.Analysis Steps 5.1 Sample Preparation 5.1.1 Baked goods, puffed foods, and fried cereal foods Take 100g of sample, add 100g of dry ice, and then grind it evenly using a tissue homogenizer. After the dry ice has completely evaporated, freeze it at -18℃. save. Note. Dry ice has an extremely low temperature; thick cotton gloves or other protective coverings (such as tongs) should be used to handle it. Dry ice sublimates and releases carbon dioxide, which can cause tissue to pulverize. If the internal pressure of the machine increases, attention should be paid to its pressure-bearing capacity to prevent the cavity from bursting due to excessive pressure, and good ventilation should be ensured. 5.1.2 Coffee, tea, sugar, infant formula and complementary foods for infants Take 100g of sample, grind it evenly with a tissue homogenizer (instant coffee and infant formula were sampled directly), and store it frozen at -18℃. Note. During the pulverization process, prevent the sample from being overheated, which could affect the measurement results. 5.2 Sample Extraction Accurately weigh 0.5 g to 2 g of the sample (accurate to 0.001 g), add 10 μL of 10 mg/L 13C3-acrylamide internal standard working solution (chromatographic analysis). Column method) or 20 μL (matrix dispersion solid phase extraction and solid phase extraction column method), then add 10 mL of water, shake for 30 min, and then at 4000 r/ Centrifuge for 10 minutes and collect the supernatant for further purification. If a supernatant cannot be obtained by centrifugation, such as for matrix samples like instant coffee or infant formula, Then take the suspension for purification. 5.3 Sample Cleaning 5.3.1 Matrix Dispersion Solid Phase Extraction (Applicable to all foods) Add 5 mL of n-hexane to the supernatant of the sample extraction, shake and extract for 5 min, then centrifuge at 10000 r/min for 5 min to remove organic matter. The phase was then extracted once more with 5 mL of n-hexane. 10 mL of acetonitrile was added to the extract, and the mixture was shaken for 10 min. 0.5 g of sodium chloride was then added. Use 4g of anhydrous sodium sulfate as a salting-out agent and shake for 5 min. Centrifuge at 10000 rpm for 5 min, and take 2.00 mL of the supernatant to disperse in a matrix. Vortex in a liquid chromatography tube for 5 minutes, centrifuge at 10000 rpm for 5 minutes, take 1 mL of the supernatant and concentrate to near dryness using a nitrogen blower, then add 0.20 mL of water. Reconstitute, filter through a 0.22 μm aqueous filter membrane, and proceed to analysis. 5.3.2 Other purification methods (applicable to foods other than coffee, tea, sugar, infant formula, and infant complementary foods) 5.3.2.1 Solid-phase extraction column method Add 5 mL of n-hexane to the supernatant of the sample extraction, shake and extract for 10 min, then centrifuge at 10000 r/min for 5 min to remove organic matter. The phase was then extracted once more with 5 mL of n-hexane. 6 mL of the extract was filtered through a 0.45 μm aqueous membrane, and the filtrate was collected for hydrophilic and hydrophilic analysis. Purification treatment of the oil equilibrium solid-phase extraction column. Before use, the hydrophilic-lipophilic equilibrium solid-phase extraction column is activated sequentially with 3 mL of methanol and 3 mL of water. [The following text appears to be unrelated and possibly a separate entry. "Take the..."] Add 5.00 mL of the filtrate to a hydrophilic-lipophilic balanced solid-phase extraction column, collect the eluent, and elute with 4 mL of 80% methanol aqueous solution. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 5009.204-2025_English be delivered?Answer: Upon your order, we will start to translate GB 5009.204-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB 5009.204-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.204-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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