GB/T 38261-2019 English PDFUS$359.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 38261-2019: Nanotechnology - Determination of silver contents in biological samples - Inductively coupled plasma mass spectrometry Status: Valid
Basic dataStandard ID: GB/T 38261-2019 (GB/T38261-2019)Description (Translated English): Nanotechnology - Determination of silver contents in biological samples - Inductively coupled plasma mass spectrometry Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A43 Classification of International Standard: 71.040.40 Word Count Estimation: 18,118 Date of Issue: 2019-12-10 Date of Implementation: 2020-07-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 38261-2019: Nanotechnology - Determination of silver contents in biological samples - Inductively coupled plasma mass spectrometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. (Nanotechnology silver content measurement in biological samples inductively coupled plasma mass spectrometry) ICS 71.040.40 A43 National Standards of People's Republic of China Measurement of silver content in nanotechnology biological samples Inductively coupled plasma mass spectrometry 2019-12-10 release 2020-07-01 implementation State Administration of Market Supervision Published by the National Standardization Management Committee ContentsForeword III Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Abbreviations 1 5 Principle 1 6 Instruments and reagents 1 7 Sample 2 8 Test step 3 9 Data analysis 4 10 Accuracy and precision 4 11 Test report 5 Appendix A (informative) Experimental Flowchart 6 Appendix B (informative) Test report template 8 Appendix C (Informative) Example of measurement of silver content in biological tissue containing silver-coated gold nanorods 9 References 13ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some elements of this document may involve patents. The issuer of this document is not responsible for identifying these patents. This standard is proposed by the Chinese Academy of Sciences. This standard is under the jurisdiction of the National Nanotechnology Standardization Technical Committee (SAC/TC279). This standard was drafted. National Nanoscience Center, China Food and Drug Inspection and Research Institute, Beijing Physical and Chemical Analysis and Testing Center, National Pharmaceutical Center for Medical Device Technical Evaluation of Product Supervision and Administration. The main drafters of this standard. Wu Meiyu, Liu Ying, Li Ruiru, Wang Xinsheng, Liu Lin, Gao Xia, Zhao Ting, Chen Kuan, Dan Mo, Chen Liang, Xu Liming, Xie Liming.IntroductionSilver nanoparticles have a broad spectrum of antibacterial activity and are widely used in daily necessities and medical devices [1,2]. With the range of applications It has been enlarged, and the ways of silver nanoparticles entering the human body have become more diversified and exist in biological tissues in various forms. So the silver exposed wind Risk has aroused great concern. In the evaluation of biological effects and safety of silver nanoparticles, such as toxicokinetics, after exposure to silver nanoparticles, It exists in biological samples in various forms such as particles and ions, and different forms of silver elements play an important role in their biological functions. Therefore, it is very important to accurately measure the total content of silver in biological samples. However, there are currently no relevant national standards. Inductively coupled plasma mass spectrometry (ICP-MS) is a mass spectrometric elemental analysis method using plasma as an ion source. Simultaneous determination of multiple elements is suitable for the analysis of trace or trace heavy metal elements [3 ~ 5]. This standard includes exposure via silver nanoparticles Microwave digestion method for biological samples, and the method for measuring the total silver content in digested samples by ICP-MS. Measurement of silver content in nanotechnology biological samples Inductively coupled plasma mass spectrometry Warning --- Some reagents used in this standard method are corrosive and strong oxidizing, you need to be careful to avoid contact with skin and clothing The microwave digestion process should be carried out in a fume hood and operated in accordance with the safety regulations of the relevant equipment of the instrument; pay attention to the safe use of electricity.1 ScopeThis standard specifies the quantitative measurement method of silver content in biological samples, including sample preparation and measurement steps. This standard applies to the quantitative measurement of silver content in a variety of biological samples (biological tissues, cells, 3D tissue models, etc.).2 Normative referencesThe following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19619 Nanomaterials terminology GB/T 30903 Determination of impurity elements in inorganic chemical products. Inductively coupled plasma mass spectrometry (ICP-MS)3 terms and definitionsThe terms and definitions defined in GB/T 19619 and GB/T 30903 apply to this document.4 AbbreviationsThe following abbreviations apply to this document. ICP-MS. inductively coupled plasma mass spectrometry5 PrincipleThe volume of biological samples was determined by microwave digestion, and the resulting solution was tested for elemental content by ICP-MS. Internal standard Method, that is, the internal standard element of the same concentration is added online by a peristaltic pump in the test sample, blank sample and spiked sample. The quantitative relationship between the ratio of the degree to the intensity value of the internal standard element and the silver element concentration enables the quantitative analysis of silver. In this method, a spiked sample is set to Determine the accuracy of the measurement results.6 Instruments and reagents6.1 Instrument 6.1.1 Inductively coupled plasma mass spectrometer (ICP-MS analyzer). 6.1.2 Microwave Digestion Device. It has a programmed power setting function, the maximum output power is ≥1200W, and the maximum setting temperature is ≥195 ° C. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 38261-2019_English be delivered?Answer: Upon your order, we will start to translate GB/T 38261-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 38261-2019_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 38261-2019_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |
