GB/T 38137-2019 English PDFUS$199.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 38137-2019: Guidelines for in vitro screening of nucleic acid aptamers Status: Valid
Basic dataStandard ID: GB/T 38137-2019 (GB/T38137-2019)Description (Translated English): Guidelines for in vitro screening of nucleic acid aptamers Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A20 Classification of International Standard: 07.080 Word Count Estimation: 10,125 Date of Issue: 2019-10-18 Date of Implementation: 2019-10-18 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 38137-2019: Guidelines for in vitro screening of nucleic acid aptamers---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Guidelines for in vitro screening of nucleic acid aptamers ICS 07.080 A20 National Standards of People's Republic of China Guidelines for in vitro screening of nucleic acid aptamers Published on.2019-10-18 2019-10-18 implementation State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the China National Institute of Standardization. This standard was drafted. Hebei Food Inspection Institute, China National Institute of Standardization, University of Science and Technology of China, Hefei University of Technology, Hebei Agricultural University, Southwest University, Jiangnan University, Henan University, Zhejiang Gongshang University, Beijing Food Science Research Institute, Hebei Medical University. The main drafters of this standard. Luo Zhaofeng, Zhou Wei, Ma Aijin, Zhang Yan, Yan, Zheng Lei, Wang Xianghong, Chen Jia, Wang Zhouping, Chen Wei, Ma Liang, Tian Yiling, Zhang Cuixia, Li Yongbo, Li Yongyan, Kang Wenyi, Wang Yanbo, Fu Linglin, Sun Yong, Lu Pin. Guidelines for in vitro screening of nucleic acid aptamers1 ScopeThis standard specifies the screening process for screening of nucleic acid aptamers in vitro, screening process monitoring, sequencing and sequence analysis, and identification of candidate aptamers. Set and optimize, anti-pollution measures. This standard is applicable to different levels of target objects such as metal ions, small molecules, macromolecules, cells and particles, tissues and mixed targets. Screening of nucleic acid aptamers.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Nucleic acid aptamer nucleicacidaptamer An oligonucleotide fragment capable of high affinity and specific binding to a specific target. Note. Targets can be metal ions, small molecules, macromolecules, cells, tissues, and mixed targets. The nucleic acid aptamer is folded into a specific three-dimensional structure, The spatial conformation complements the high specific binding of the target with high affinity, and is mainly composed of a short single-stranded nucleotide sequence (single-stranded DNA or RNA). 3.2 In vitro screening technology invitroscreeningtechnique The formed target-oligonucleotide complex and free oligonucleotide are formed by interaction of the target with a large-capacity random oligonucleotide library Isolation, and then using the PCR in vitro amplification technique to amplify the oligonucleotide forming the complex, and repeating the above screening steps multiple times to finally obtain A technique for nucleic acid aptamers that specifically bind to a target.4 AbbreviationsThe following abbreviations apply to this document. PAGE. polyacrylamide gel electrophoresis; PCR. polymerase chain reaction (polymerase chain reaction); polyA. polyadenylation; SELEX. Exponentially enriched ligand system evolution (exponentialyenrichedligandsystemevolution).5 screening process5.1 Screening basic ways The basic approach of SELEX technology is to store large-capacity random oligonucleotide sequence libraries (1013~1015 random oligonucleotide sequences) with targets. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 38137-2019_English be delivered?Answer: Upon your order, we will start to translate GB/T 38137-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 38137-2019_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 38137-2019_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |
