GB/T 37115-2018 English PDF

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GB/T 37115-2018: Protocol of diagnosis for infection with abalone herpes virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 37115-2018	279	Add to Cart	3 days	Protocol of diagnosis for infection with abalone herpes virus	Valid

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Basic data

Standard ID: GB/T 37115-2018 (GB/T37115-2018)
Description (Translated English): Protocol of diagnosis for infection with abalone herpes virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 14,170
Date of Issue: 2018-12-28
Date of Implementation: 2019-07-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 37115-2018: Protocol of diagnosis for infection with abalone herpes virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for infection with abalone herpersvirus ICS 65.020.30 B41 National Standards of People's Republic of China Diagnosis of alopecia virus disease Published on.2018-12-28 2019-07-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization does not assume responsibility for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. Shenzhen Institute of Inspection and Quarantine, National Aquatic Technology Extension Station, Shenzhen Customs of the People's Republic of China. The main drafters of this standard. Wang Jinjin, Li Qing, Yu Weizhong, Liu Wei, Liu Ying, Shi Xiujie, Zheng Xiaocong, He Junqiang, Yu Li, Jia Peng, Lan Wensheng. Diagnosis of alopecia virus disease

1 Scope

This standard gives the clinical symptoms of alopecia virus disease, specifies the histopathological detection method of alopecia virus disease, real-time fluorescence polymerization Enzyme chain reaction detection method, polymerase chain reaction detection method. This standard applies to the epidemiological investigation, monitoring, diagnosis and quarantine of alopecia virus disease.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 18088 entry and exit animal quarantine sampling SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. AbHV. Abalone Herpesvirus CTAB. CetyltrimethylAmmonium Bromide Ct value. Cycle threshold (Cyclethreshold) EB. Ethidium Bromide EDTA. Ethylene Diamine Tetraacetic Acid PCR. Polymerase Chain Reaction (PolymeraseChainReaction) qPCR. real-time fluorescent polymerase chain reaction (quantitativereal-timePolymeraseChainReaction)

4 reagents and materials

4.1 Unless otherwise stated, the biochemical reagents used in this standard are analytically pure reagents. 4.2 If not specified, the test water should meet the specifications of GB/T 6682 water. 4.3 Taq enzyme and 10 times Taq enzyme concentration buffer; Taq enzyme concentration is 5U/μL, stored at -20 ° C, to avoid repeated freezing and thawing. 4.4 dNTPs. containing 10 cmoL/L of dCTP, dGTP, dATP and dTTP. 4.5 qPCR primers and probes. AbHVORF66F1. 5'-CCCGGACACCAGTAAGAAC-3' AbHVORF66R1. 5'-AGGCTGCTATGCGTATGA-3' Probe (AbHV66Prb1). 5'-6FAM-TGGCCGTCGAGATGTCCATG-TAMRA-3' A 146 bp fragment of the ORF66 gene was amplified for fluorescent PCR. (See Appendix A) AbHVORF77F1. 5'-AACCACTTGTTCGGGTTCT-3' AbHVORF77R1. 5'-AGGGTGATTAATGCGGAGT-3' ......

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