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GB/T 37077-2018 English PDF

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US$119.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 37077-2018: Determination of cholesterol in eggs -- Enzymatic method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 37077-2018	119	Add to Cart	3 days	Determination of cholesterol in eggs -- Enzymatic method	Valid

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Basic data

Standard ID: GB/T 37077-2018 (GB/T37077-2018)
Description (Translated English): Determination of cholesterol in eggs -- Enzymatic method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 6,632
Date of Issue: 2018-12-28
Date of Implementation: 2019-07-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 37077-2018: Determination of cholesterol in eggs -- Enzymatic method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of cholesterol in eggs--Enzymatic method ICS 67.050 X04 National Standards of People's Republic of China Determination of cholesterol in eggs - Enzymatic method Published on.2018-12-28 2019-07-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Husbandry Standardization Technical Committee (SAC/TC274). This standard was drafted. Ministry of Agriculture and Rural Poultry Quality Supervision and Testing Center (Yangzhou), Institute of Poultry, Chinese Academy of Agricultural Sciences. The main drafters of this standard. Gao Yushi, Ge Qinglian, Ma Lina, Tang Xiujun, Chen Dawei, Liu Yinyin, Zhang Jing, Zhang Xiaoyan, Pu Junhua, Tang Mengjun, Fan Yanfeng, Gu Rong. Determination of cholesterol in eggs - Enzymatic method

1 Scope

This standard specifies the method for the determination of cholesterol in poultry eggs by enzymatic method. This standard applies to eggs, duck eggs, goose eggs, pigeon eggs, quail eggs and other fresh eggs. The detection limit of this standard method is 1mg/100g, and the limit of quantification is 3mg/100g.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

Cholesterol esters are hydrolyzed to free cholesterol and fatty acids by cholesterol esterase, and cholesterol is produced by cholesterol oxidase. △4-cholestenone and H2O2, H2O2 reacted with 4-aminoantipyrine and phenol to form red quinone imine by oxidation enzyme. The absorbance of the sample was measured at a wavelength of 505 nm, and the absorbance of the quinone imine was proportional to the amount of cholesterol in the sample. According to its absorbance and standard The curve is compared or the cholesterol content of the sample is calculated from the standard curve regression equation.

4 reagents or materials

Unless otherwise stated, the reagents used in this standard are of analytical grade and the water is the third grade water specified in GB/T 6682. 4.1 Methanol (CH3OH). 4.2 Ether (C4H10O). 4.3 Anhydrous ethanol (C2H6O). 4.4 Potassium hydroxide (KOH). 4.5 Sodium chloride (NaCl). 4.6 Cholesterol esterase (EC 3.1.1.13). derived from microorganisms (such as Pseudomonas, Candida), glucose oxidase and uricase content Less than 0.01%, no catalase, specific activity of about 100kU/g enzyme protein (25 ° C, cholesterol oleate as a substrate). 4.7 Cholesterol oxidase (EC1.1.1.6). derived from microorganisms (such as Nocardia, Streptomyces cocci), glucose oxidase and uricase The amount is less than 0.01%, does not contain catalase, and has a specific activity of about 45 kU/g enzyme protein (37 ° C, cholesterol as a substrate). 4.8 Peroxidase (EC1.11.1.9). derived from horseradish, purity value (Reinheitszah1 ratio, A403nm/A275nm) is about 3.0, specific activity The enzyme 1000 kU/g enzyme protein [25 ° C, 2, 2 '-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) as a substrate]. 4.9 Cholesterol (C27H46O, CAS No.. 57-88-5). purity ≥ 99%. 4.10 Potassium Hydroxide Solution (600g/L). Weigh 600g of potassium hydroxide, dissolve it with water and dilute to 1000mL, shake well. 4.11 Sodium chloride solution (50g/L). Weigh 50g of sodium chloride, add water to dissolve and dilute to 1000mL, shake. 4.12 Disodium hydrogen phosphate solution (0.3mol/L). Weigh 107.4g disodium hydrogen phosphate (Na2HPO4·12H2O), dissolve with water and make up to volume Shake to 1000 mL. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 37077-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 37077-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 37077-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 37077-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.