GB/T 36875-2018 English PDFUS$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36875-2018: Method of RT-nPCR for detection of classical swine fever virus Status: Valid
Basic dataStandard ID: GB/T 36875-2018 (GB/T36875-2018)Description (Translated English): Method of RT-nPCR for detection of classical swine fever virus Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 10,151 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36875-2018: Method of RT-nPCR for detection of classical swine fever virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Method of RT-nPCR for detection of classical swine fever virus ICS 11.220 B41 National Standards of People's Republic of China Swine fever virus RT-nPCR detection method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, China Veterinary Drug Inspection Institute. The main drafters of this standard. Guo Huancheng, Wang Qin, Xu Wei, Feng Wei, Zhang Qianyi, Zhao Qizu, Zou Xingqi, Zhu Yuanyuan, Tu Changchun. Swine fever virus RT-nPCR detection method1 ScopeThis standard specifies the technical requirements for the swine fever virus-specific reverse transcription-sleeve polymerase chain reaction (RT-nPCR) method. This standard applies to the detection of swine fever virus nucleic acid in pig organs, blood, feces and cell cultures that may be infected with swine fever virus. Diagnosis and monitoring of swine fever. This standard of swine fever virus RT-nPCR method cannot distinguish between infected swine fever virus wild strain and immunized vaccine strain.2 AbbreviationsThe following abbreviations apply to this document. DEPC. diethylpyrocarbonate EDTA-2Na. ethylenediaminetetraacetic aciddisodiumsalt RNA. ribonucleic acid RT-nPCR. reverse transcription-nested polymerase chain reaction (reversetranscriptionandnestedpolymerasechainreac- Tion)3 Preparation before the experiment3.1 Laboratory conditions 3.1.1 Laboratory partition The PCR laboratory partition includes a nucleic acid extraction region, a PCR reaction system preparation region---dispensing region, an amplification region, and an electrophoresis region. The sequence of processes is Nucleic acid extraction zone→displacing zone→amplification zone→electrophoresis zone. Dedicated to instruments and consumables in each area. 3.1.2 Instruments and equipment to be equipped in the laboratory 3.1.2.1 Instruments. Class II biosafety cabinet, desktop high speed refrigerated centrifuge, ordinary refrigerator (-20 ° C and 4 ° C), analytical balance, PCR amplification Instrument, electrophoresis, electrophoresis tank, UV gel imager (or UV analyzer), tissue grinder, tonsil sampler, microwave oven, single channel transfer Liquid filter (2 μL, 10 μL,.200 μL, 1000 μL). 3.1.2.2 Equipment. sterile syringe, ophthalmic scissors, ophthalmology, weighing paper, cotton swabs, sterilized toothpicks, 500mL measuring cylinders, 500mL conical flasks, One-time RNase-free tips (10 μL,.200 μL, 1000 μL), 1.5 mL RNase-free tubes, and 0.2 mL thin-wall PCR tubes. 3.2 Chemicals and reagents 3.2.1 Total RNA extraction reagent Trizol. 3.2.2 Preparation of TAE electrophoresis buffer, see Appendix A for preparation methods. 3.2.3 1% agarose gel, see Appendix A for the preparation method. 3.2.4 Other reagents..200U/μLM-MLV reverse transcriptase, 5×M-MLV buffer, 40U/μL RNase inhibitor, DEPC- H2O, 5U/μLExTaq DNA polymerase, 10×ExTaq buffer (Mg2 Plus), 6 base random primer (50 μmol/L), 6× loading buffer, dNTPs (2.5mmol/L/dNTP), isopropanol, chloroform, 75% ethanol, ddH2O, ethidium bromide instead ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36875-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36875-2018_English as soon as possible, and keep you informed of the progress. 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