| GB/T 36873-2018 English PDFUS$279.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36873-2018: Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock Status: Valid 
 Basic dataStandard ID: GB/T 36873-2018 (GB/T36873-2018)Description (Translated English): Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 14,190 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36873-2018: Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock ICS 11.220 B41 National Standards of People's Republic of China Purification test procedure for avian leukemia in the original chicken flock Breederflock Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Shandong Agricultural University, China Center for Animal Health and Epidemiology, Anhui Institute of Science and Technology, Yangzhou University, South China Agriculture University. The main drafters of this standard. Zhao Peng, Cui Zhizhong, Li Weihua, Zhang Xunhai, Qin Aijian, Ye Jianqiang, Cao Weisheng. Purification test procedure for avian leukemia in the original chicken flock1 ScopeThis standard specifies the purification test procedures for avian leukemia in the original chicken flock. This standard applies to the purification of avian leukemia in the original flocks.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 26436-2010 Avian leukemia diagnosis technology NY/T 680-2003 Avian leukosis virus p27 antigen enzyme-linked immunosorbent assay3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Original breed flock pedigreebreederflock The original flock of breeding species is selected. 3.2 Animal disease purification animaldiseaseeradication Discover diseased animals or infected animals through continuous testing and monitoring in a farm or designated area, and eliminate the poisoned animals To achieve the process of eradicating an animal disease.4 testing procedures4.1 Detection and elimination of meconium in shelled chicks 4.1.1 Collection of samples Place 0.5 mL of sterile phosphate buffered saline (phosphatebuffersaline) in each tube in a sterile 1.5 mL centrifuge tube. PBS, 0.01mol/L, pH 7.4) (see Appendix A for PBS formulation specifications), or commercial reagents in accordance with NY/T 680-2003 For the special dilution of the box, align the tube with the centrifuge tube and gently press the belly of the chicken to squeeze the meconium into a 1.5 mL centrifuge tube. 4.1.2 Sample processing and testing The 1.5 mL centrifuge tube containing meconium was thoroughly shaken and mixed on a vortex shaker, then placed in liquid nitrogen for rapid freezing and freezing. After 2 minutes, take it out and melt it in a 37 ° C water bath, then mix the tube thoroughly on the vortex shaker. So repeatedly frozen After thawing, place the centrifuge tube in a tabletop centrifuge, centrifuge at 12000g for 1min, and take the supernatant after centrifugation. It should be in accordance with NY/T 680-2003. Or use the commercial kits in accordance with NY/T 680-2003 to detect the avian leukosis virus p27 antigen in meconium. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36873-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36873-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 36873-2018_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 36873-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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