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GB/T 36873-2018 English PDF

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GB/T 36873-2018: Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 36873-2018279 Add to Cart 3 days Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock Valid

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Basic data

Standard ID: GB/T 36873-2018 (GB/T36873-2018)
Description (Translated English): Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,190
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36873-2018: Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of practice for detection of avian leukosis infection in chicken pedigree breeder flock ICS 11.220 B41 National Standards of People's Republic of China Purification test procedure for avian leukemia in the original chicken flock Breederflock Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Shandong Agricultural University, China Center for Animal Health and Epidemiology, Anhui Institute of Science and Technology, Yangzhou University, South China Agriculture University. The main drafters of this standard. Zhao Peng, Cui Zhizhong, Li Weihua, Zhang Xunhai, Qin Aijian, Ye Jianqiang, Cao Weisheng. Purification test procedure for avian leukemia in the original chicken flock

1 Scope

This standard specifies the purification test procedures for avian leukemia in the original chicken flock. This standard applies to the purification of avian leukemia in the original flocks.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 26436-2010 Avian leukemia diagnosis technology NY/T 680-2003 Avian leukosis virus p27 antigen enzyme-linked immunosorbent assay

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Original breed flock pedigreebreederflock The original flock of breeding species is selected. 3.2 Animal disease purification animaldiseaseeradication Discover diseased animals or infected animals through continuous testing and monitoring in a farm or designated area, and eliminate the poisoned animals To achieve the process of eradicating an animal disease.

4 testing procedures

4.1 Detection and elimination of meconium in shelled chicks 4.1.1 Collection of samples Place 0.5 mL of sterile phosphate buffered saline (phosphatebuffersaline) in each tube in a sterile 1.5 mL centrifuge tube. PBS, 0.01mol/L, pH 7.4) (see Appendix A for PBS formulation specifications), or commercial reagents in accordance with NY/T 680-2003 For the special dilution of the box, align the tube with the centrifuge tube and gently press the belly of the chicken to squeeze the meconium into a 1.5 mL centrifuge tube. 4.1.2 Sample processing and testing The 1.5 mL centrifuge tube containing meconium was thoroughly shaken and mixed on a vortex shaker, then placed in liquid nitrogen for rapid freezing and freezing. After 2 minutes, take it out and melt it in a 37 ° C water bath, then mix the tube thoroughly on the vortex shaker. So repeatedly frozen After thawing, place the centrifuge tube in a tabletop centrifuge, centrifuge at 12000g for 1min, and take the supernatant after centrifugation. It should be in accordance with NY/T 680-2003. Or use the commercial kits in accordance with NY/T 680-2003 to detect the avian leukosis virus p27 antigen in meconium.
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