GB/T 36871-2018 English PDF

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GB/T 36871-2018: Multiplex RT-PCR to detect porcine transmissible gastroenteritis virus, porcine epidemic diarrhoea virus and porcine rotavirus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36871-2018	279	Add to Cart	3 days	Multiplex RT-PCR to detect porcine transmissible gastroenteritis virus, porcine epidemic diarrhoea virus and porcine rotavirus	Valid

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Basic data

Standard ID: GB/T 36871-2018 (GB/T36871-2018)
Description (Translated English): Multiplex RT-PCR to detect porcine transmissible gastroenteritis virus, porcine epidemic diarrhoea virus and porcine rotavirus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,191
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36871-2018: Multiplex RT-PCR to detect porcine transmissible gastroenteritis virus, porcine epidemic diarrhoea virus and porcine rotavirus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Multiplex RT-PCR to detect porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus ICS 11.220 B41 National Standards of People's Republic of China Porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and Porcine rotavirus multiplex RT-PCR detection method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Huazhong Agricultural University, China Center for Animal Health and Epidemiology. The main drafters of this standard. He Qigai, Ku Xugang, Zhang Kun, Chen Fangzhou, Fan Shengxian, Meng Xianrong, Chen Pin, Jiang Wen, Chen Huanchun. Porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and Porcine rotavirus multiplex RT-PCR detection method

1 Scope

This standard specifies the porcine transmissible gastroenteritis virus (TGEV), pig epidemic Multiple porcine epidemic virus (PEDV) and porcinerotavirus (PoRV) RT-PCR detection method. This standard applies to the detection of porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus.

2 Abbreviations

The following abbreviations apply to this document. TGEV. porcine transmissible gastroenteritisvirus PEDV. Porcine epidemic diarrhea virus (porcineepidemicdiarrheavirus) PoRV. Porcine Rotavirus (porcinerotavirus) RT-PCR. reverse transcription-polymerase chain reaction (reversetranscription-polymerase chain reaction) PBS. phosphate buffer (phosphatebuffersaline) TAE. electrophoresis buffer (tris-acetate-ethylenediaminetetraacetic acidbuffer) TCID50. half of the cell infection volume (mediantissuecultureinfectivedose) RNA. ribonucleic acid DEPC. diethylpyrocarbonate

3 reagents and equipment

The reagents used were of analytical grade unless otherwise stated. The reagents used to extract the RNA should be dispensed using an RNase-free container. 3.1 Instrument 3.1.1 Biological safety cabinet. 3.1.2 High speed refrigerated centrifuge. 3.1.3 Tissue homogenizer or mortar. 3.1.4 PCR instrument. 3.1.5 Horizontal electrophoresis tank. 3.1.6 Gel imaging system. 3.1.7 UV Transmitter. 3.1.8 2 ° C ~ 8 ° C refrigerator. 3.1.9 -20 ° C refrigerator. 3.1.10 Single-channel micropipette (0.5μL~10μL, 10μL~50μL, 20μL~200μL, 100μL~1000μL, etc.). 3.2 Reagents 3.2.1 Chloroform, stored at room temperature. ......

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