GB/T 36853-2018 English PDF

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GB/T 36853-2018: Detection and identification of Pseudomonas syringae pv. lachrymans
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36853-2018	279	Add to Cart	3 days	Detection and identification of Pseudomonas syringae pv. lachrymans	Valid

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Basic data

Standard ID: GB/T 36853-2018 (GB/T36853-2018)
Description (Translated English): Detection and identification of Pseudomonas syringae pv. lachrymans
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,165
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36853-2018: Detection and identification of Pseudomonas syringae pv. lachrymans

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pseudomonas syringae pv.lachrymans ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of cucumber bacterial keratosis Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, the People's Republic of China Huangdao Entry-Exit Inspection and Quarantine Bureau, the Chinese people And the National Yantai Exit-Exit Inspection and Quarantine Bureau, Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, and Biotechnology Research of the Chinese Academy of Agricultural Sciences Institute of Nanjing Agricultural University. Drafters of this standard. Tian Wei, Xia Mingxing, Zhao Wenjun, Su Zhiping, Huang Yingbo, Li Weimin, Hu Baishi, Tian Yanli. Method for quarantine and identification of cucumber bacterial keratosis

1 Scope

This standard specifies the methods for sample preparation, isolation and culture, immunology and molecular biology of cucumber bacterial leaf spot bacteria. This standard is applicable to the quarantine and identification of cucumber seeds, plants and their products that may carry Cucumber.

2 Basic information of cucumber bacterial leaf spot bacteria

Chinese name. Cucumber bacterial leaf spot, Cucumber leaf spot Scientific name. Pseudomonassyringaepv.lachrymans (Smith Synonym. Bacilluslachrymans (Smith Burgvits1935, BacteriumlachrymansSmith An)Patel Monasburgeri (Potebnya) Korobko Carsner1918, Pseudomonaslachrymansf.cucumisGorlenko1961 English name. Cucurbitangularleafspot Classification status. Bacteria, Proteobacteria, γ-Proteobacteria, False Pseudomonadales, Pseudomonadaceae, Pseudomonas. Transmission route. The carrier seeds are the main way for long-distance transmission of pathogenic bacteria. The pathogens invade the host from the stomata, wounds and water holes. Water, wind and rain, air currents, insects and farming operations spread across the field. See Appendix A for additional information on cucumber bacterial leaf spot bacteria.

3 Principle of the method

Immunological detection according to the specific reaction between cucumber bacterial leaf spot bacteria and antibodies; according to cucumber bacterial leaf spot bacteria Specific DNA sequence for molecular biological detection; culture traits, biological characteristics, host range according to cucumber bacterial leaf spot And pathogenic bacteria are isolated and cultured and pathogenicity is determined.

4 instruments and main reagents

4.1 Instruments and equipment PCR instrument, real-time fluorescence PCR instrument, ultra-clean workbench, autoclave, constant temperature incubator, ice machine, high-speed refrigerated centrifuge, table Small centrifuges, ultra-low temperature refrigerators, conventional refrigerators, vortex shakers, micro-injectors, electrophoresis, gel imaging systems. 4.2 Primary reagents All reagents were of analytical grade unless otherwise stated. See Appendix B and Appendix C for reagents required for molecular biology testing. Nutritional agar (NA) medium preparation method. beef extract 3.0g, peptone 5.0g, yeast extract 1.0g, glucose 10.0g, agar 15.0 g, add double distilled water to 1000 mL, adjust the pH to 7.0, and moist heat sterilization (121 ° C, 15 min). ......

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