GB/T 36852-2018 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 36852-2018: Detection and identification of Erwinia pyrifoliae Status: Valid
Basic dataStandard ID: GB/T 36852-2018 (GB/T36852-2018)Description (Translated English): Detection and identification of Erwinia pyrifoliae Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,112 Date of Issue: 2018-09-17 Date of Implementation: 2019-04-01 Regulation (derived from): National Standard Announcement No. 11 of 2018 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 36852-2018: Detection and identification of Erwinia pyrifoliae---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Erwinia pyrifoliae ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of Asian pear fire blight Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The structure does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Nanjing Agricultural University, Shanghai Entry and Exit Inspection and Quarantine Bureau, Zhejiang University, Yantai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhao Wenjun, Tian Hao, Hu Baishi, Tian Yanli, Yi Jianping, Lou Binggan, Li Hongye, Ge Quanqing. Method for quarantine and identification of Asian pear fire blight1 ScopeThis standard specifies the methods for the isolation, culture, immunology and molecular biology of P. acerifolia. This standard applies to the quarantine and identification of P. oleracea in pear seedlings, rootstocks, scions and young fruit transmissions, as well as field investigations of diseases and monitor. 2 Basic information on P. oxysporum Chinese name. Asian Phytophthora Scientific name. ErwiniapyrifoliaeKimetal. English name. ShootblightofAsianpear Classification status. Bacteria, Proteobacteria, Gammaproteobacteria, Intestines Enterobacteriales, Enterobacteriaceae, Erwinia. Routes of transmission. propagation materials of infected pears (including seedlings, rootstocks and scions), insects, wind, rain, and contaminated packaging materials and transport Many natural and human factors, such as the loss of tools, can cause the spread of Asian pear fire blight. Among them, the breeding material of the infected pear is Asia. The main route of long-distance transmission of pear fire blight. See Appendix A for additional information on P. oxysporum.3 Principle of the methodImmunological detection according to the specific reaction between P. oxysporum and antibodies; according to the specificity of P. oxysporum DNA sequence for molecular biological detection; according to the culture traits, biological characteristics, host range and hazard symptoms of P. oxysporum Isolation, culture and pathogenicity determination of pathogenic bacteria.4 instruments and main reagents4.1 Instruments and equipment Ultra-clean workbench, constant temperature incubator, shaker, ultra-low temperature refrigerator, conventional refrigerator, autoclave, constant temperature water bath, small centrifuge, high speed Refrigerated centrifuge, microscope, PCR instrument, real-time fluorescence PCR instrument, electrophoresis instrument, horizontal electrophoresis tank, gel imaging system, ice machine, electronic day Flat, vortex shaker, micro-injector, oven, etc. 4.2 Primary reagents All reagents were of analytical grade unless otherwise stated. See Appendix B and Appendix C for reagents required for molecular biology testing.5 Quarantine and identification methods5.1 symptom check Refer to the symptom description in Appendix A to check whether the plant has typical symptoms of Asian pear fire blight, mainly check the young shoots, flowers, young fruits and branches. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36852-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36852-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 36852-2018_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 36852-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |
