GB/T 36848-2018 English PDF

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GB/T 36848-2018: Detection and identification of Avocado sunblotch viroid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36848-2018	199	Add to Cart	3 days	Detection and identification of Avocado sunblotch viroid	Valid

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Basic data

Standard ID: GB/T 36848-2018 (GB/T36848-2018)
Description (Translated English): Detection and identification of Avocado sunblotch viroid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 10,125
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36848-2018: Detection and identification of Avocado sunblotch viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Avocado sunblotch viroid ICS 65.020.01 B16 National Standards of People's Republic of China Avocado spot plaque virus quarantine and identification method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people Heguo Shandong Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Zhang Yongjiang, Zheng Yi, Zhang Jingyu, Xin Yanyan, Xiang Jun, Lu Xiaoyu. Avocado spot plaque virus quarantine and identification method

1 Scope

This standard specifies avocado plaque virus reverse transcriptase polymerase chain reaction (RT-PCR) and reciprocating bidirectional polyacrylamide gel electrophoresis Quarantine identification method. This standard applies to the quarantine and identification of plants and their fruits that may carry avocado plaque.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Basic information

Chinese name. Avocado plaque virus Scientific name. Avocadosunblotchviroid Abbreviation. ASBVd Classification status. Avsunviroidae Avsunviroid. See Appendix A for additional information on avocado plaques.

4 Principle of the method

Preliminary determination based on the symptoms of ASBVd on host plants; based on its natural state of RNA and different structural molecules after denaturation Reciprocating two-way polyacrylamide gel electrophoresis (Return-Polyacrylamide) in the difference of mobility in polyacrylamide gel electrophoresis GelElectrophoresis, R-PAGE); RT-PCR detection based on genomic characteristics, results by electrophoresis band size determination. 5 Equipment and facilities, appliances and reagents 5.1 Equipment and facilities Balance (sensitivity 0.001g), high-speed refrigerated centrifuge, pH meter, PCR instrument, electrophoresis instrument, electrophoresis tank, gel imaging system, isolation and quarantine temperature Room, constant temperature water bath, low temperature refrigerator, etc. Micropipettes (2 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL), mortar, centrifuge tubes (1.5 mL, 2.0 mL), pots, Disinfect the soil and so on. 5.2 RT-PCR detection reagent Unless otherwise specified, all reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. See Appendix B for details. ......

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