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GB/T 36847-2018 English PDF

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GB/T 36847-2018: Detection and identification of Acidovorax cattleyae
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 36847-2018279 Add to Cart 3 days Detection and identification of Acidovorax cattleyae Valid

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Basic data

Standard ID: GB/T 36847-2018 (GB/T36847-2018)
Description (Translated English): Detection and identification of Acidovorax cattleyae
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,166
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36847-2018: Detection and identification of Acidovorax cattleyae

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Acidovorax cattleyae ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of orchid brown spot disease Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Gansu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese People And the National Erlianhot Entry-Exit Inspection and Quarantine Bureau, Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Tianjin Entry and Exit of the People's Republic of China Inspection and Quarantine Bureau, Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhao Wenjun, Wang Xiqiao, Zhang Yonghong, Tian Wei, inch Dongyi, Liao Fang, Li Yan. Method for quarantine and identification of orchid brown spot disease

1 Scope

This standard specifies the isolation and culture of orchid brown spot pathogen, immunology and molecular biology detection and identification methods. This standard applies to the quarantine and identification of orchid brown spot disease in orchid plants and the field investigation and monitoring of the disease.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. Quarantine Regulations for SN/T 1157 Entry and Exit Plant Seedlings

3 Basic information on orchid brown spot disease

Chinese name. Orchid brown spot pathogen, orchid bacterial brown spot disease Scientific name. Acidovoraxcattleyae (Pavarino1911) Schaadetal.2009 Synonym. Acidovoraxavenaesubsp.cattleyae (Pavarino1911) Wilemsetal.1992 Pseudomonascattleyae (Pavarino1911) Savulescu1947 Bacilluscattleyae (Pavarino) Stapp1928 BacteriumcattleyaePavarino1911 Phytobacteriumcattleyae(Pavarino)Magrou Phytomonascattleyae (Pavarino) Ark English name. Bacterialbrownspotoforchid Classification status. Bacteria, Proteobacteria, Betaproteobacteria, Burkholderia Burkholderiales, Comonmonadaceae, Acidovorax. Transmission route. Long-distance transmission mainly relies on the seedlings, and the short-distance propagation mainly spreads the pathogens by means of foliar watering, spraying or fertilization. Sowing on healthy plants, infecting through leaf wounds and natural orifices, causing secondary infection. See Appendix A for additional information on orchid brown spot.

4 Principle

Immunological detection of plants or leaves according to the specific reaction between orchid leaf spot and antibody; according to orchid brown spot Specific DNA sequences for molecular biological testing; culture traits, biological characteristics, host range and hazards of orchid leaf spot Isolation, culture and pathogenicity of pathogenic bacteria such as symptoms.

5 instruments and main reagents

5.1 Instruments and equipment PCR instrument, real-time fluorescence PCR instrument, microscope, ultra-clean workbench, constant temperature incubator, sterilizer, ice machine, high-speed refrigerated centrifuge, table
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