GB/T 36841-2018 English PDF

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GB/T 36841-2018: Detection and identification of Peach rosette mosaic virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36841-2018	279	Add to Cart	3 days	Detection and identification of Peach rosette mosaic virus	Valid

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Basic data

Standard ID: GB/T 36841-2018 (GB/T36841-2018)
Description (Translated English): Detection and identification of Peach rosette mosaic virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,140
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36841-2018: Detection and identification of Peach rosette mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Peach rosette mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of peach cluster mosaic virus Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, the People's Republic of China Nanhai Entry-Exit Inspection and Quarantine Bureau, the Chinese people Heguo Xiamen Entry-Exit Inspection and Quarantine Bureau, Yantai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang University. The main drafters of this standard. Zhang Yongjiang, Qiu Yanhong, Tan Wei, Liao Furong, Xiang Jun, Li Jinqing, Wu Jianxiang, Xin Yanyan. Method for quarantine and identification of peach cluster mosaic virus

1 Scope

This standard specifies the serological and molecular biological detection methods for quarantine identification of peach cluster mosaic virus. This standard applies to the quarantine and identification of plant materials such as peach trees, grapes, plums, plums and other mediators such as the nematode clusters of mosaic virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Basic information

Chinese name. Peach Cluster Mosaic Virus Scientific name. Peachrosettemosaicvirus Abbreviation. PRMV Taxonomic status. associated with the genus Cocoviridae, Comovirinae, nematode polyhedrosis virus (Nepovirus). See Appendix A for additional information on Peach Clover Mosaic Virus.

4 Principle of the method

The serological and genomic characteristics of the peach cluster mosaic virus is the basis for the quarantine and identification of the virus. segment. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-) for plant samples based on specific reaction between PRMV and antibody ELISA) and dot-enzyme-linked immunosorbent assay (Dot-ELISA); reverse transcription polymerase chain reaction based on genomic characteristics of PRMV Should be (RT-PCR) detection.

5 instruments and reagents

5.1 Instrument Electronic balance (sensitivity 0.001g), high-speed refrigerated centrifuge, small instantaneous centrifuge, ordinary refrigerator, ultra-low temperature refrigerator (-80 ° C), ice making Machine, vortex oscillator, magnetic stirrer, autoclave, pH meter, ultra-clean workbench, PCR instrument, microwave oven, electrophoresis, electrophoresis tank, gel Like analyzers, microplate readers, plate washers, thermostatic water baths. 5.2 Appliances Enzyme-linked plate, adjustable pipette (2.5μL, 10μL, 20μL, 100μL,.200μL, 1000μL) and adjustable pipette tip, centrifuge tube (1.5 mL, 2.0 mL), mortar, micro-grinding, etc. ......

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