GB/T 36840-2018 English PDF

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GB/T 36840-2018: Detection and identification of Clavibacter michiganensis subsp. nebraskensis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36840-2018	279	Add to Cart	3 days	Detection and identification of Clavibacter michiganensis subsp. nebraskensis	Valid

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Basic data

Standard ID: GB/T 36840-2018 (GB/T36840-2018)
Description (Translated English): Detection and identification of Clavibacter michiganensis subsp. nebraskensis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,13
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36840-2018: Detection and identification of Clavibacter michiganensis subsp. nebraskensis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Clavibacter michiganensis subsp.nebraskensis ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of wilt disease in maize Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine, and the Chinese People And the Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Gao Wenna, Zheng Chunsheng, Luo Weifeng, Zhao Wenjun, Yi Jianping, Zhang Lijie, Bian Yong. Method for quarantine and identification of wilt disease in maize

1 Scope

This standard specifies the quarantine and identification methods for Clavibacter michiganensis subsp. nebraskensis. This standard is applicable to the quarantine and identification of corn wilt pathogens in corn seeds and corn plant materials.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 Basic information

Chinese name. Corn Phytophthora Scientific name. Clavibactermichiganensissubsp.nebraskensis (Vidaver Abbreviation. CMN English name. Goss'swilt, Goss'swiltofmaize, Goss'sbacterialwiltandleafblight, blightofmaize, Leaffrecklesofmaize,wiltofmaize,Nebraskaleaffrecklesandwilt,leaffrecklesandwilt Classification status. Bacteria, actinobacteria, Micrococcales, Mi-Cytobacteria Mi- Crobacteriaceae, Clavibacter. See Appendix A for additional information on the wilting bacteria in the corn.

4 Principle

The symptom characteristics, colony morphology, physiological and biochemical characteristics, PCR reaction and pathogenicity test results of the wilting bacteria in the corn The basis for the identification method.

5 main reagents and equipment

5.1 main reagent All reagents are either analytical or biochemical reagents unless otherwise stated. Sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, methanol, ethanol, beef extract, polyprotein 胨, yeast extract, sucrose, agar, glucose. See Appendix B for media and buffer formulations. 5.2 Instruments and equipment Microscope, clean bench, electronic balance, autoclave, refrigerator, pipette, centrifuge, incubator, PCR instrument, electrophoresis, gel imaging System, real-time fluorescence PCR instrument, Biolog identification instrument, etc. ......

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