GB/T 36822-2018 English PDF

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GB/T 36822-2018: Detection and identification of Acidovorax citrulli
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36822-2018	359	Add to Cart	4 days	Detection and identification of Acidovorax citrulli	Valid

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Basic data

Standard ID: GB/T 36822-2018 (GB/T36822-2018)
Description (Translated English): Detection and identification of Acidovorax citrulli
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 18,174
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36822-2018: Detection and identification of Acidovorax citrulli

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Acidovorax citrulli ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of melon fruit spot bacteria Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. China Academy of Inspection and Quarantine, Shenzhen Institute of Inspection and Quarantine, Nanjing Agricultural University, Chinese people Xinjiang Entry-Exit Inspection and Quarantine Bureau, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, National Melon Engineering Technology Research Center, Jiangsu Province Plant protection phytosanitary station. The main drafters of this standard. Zhao Wenjun, Feng Jianjun, Tian Hao, Tian Yanli, Zhang Xianglin, Zhao Tingchang, Hu Baishi, Gong Weirong. Method for quarantine and identification of melon fruit spot bacteria

1 Scope

This standard specifies the symptom observation, isolation culture, immunology and molecular biological detection methods of melon fruit spot bacteria. This standard is applicable to the quarantine and identification of plants and seeds of Cucurbitaceae crops such as watermelon and melon which may have melons.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. Quarantine and identification method of SN/T 1465 watermelon bacterial fruit spot disease

3 Basic information on melons

Scientific name. Acidovoraxcitrulli (Schaadetal.) Schaadetal.,.2008 Synonym. Acidovoraxavenaesubsp.citrulli (Schaadetal., 1978) Wilemsetal.,.1992 Pseudomonasavenaesubsp.citrulli (Schaadetal., 1978) Huetal.,.1991 Pseudomonaspseudoalcaligenessubsp.citrulliSchaadetal., 1978 Transmission route. It is mainly transmitted by long-distance transmission with bacteria seeds. The field diffusion mainly depends on rainwater, farming operations and insect transmission. See Appendix A for additional information on melons.

4 Principle of the method

Separating and cultivating the pathogenic bacteria according to the culture traits, biological characteristics, host range and harmful symptoms of the genus Phytophthora; according to The specific reaction between the genus Phytophthora and the antibody is immunologically detected; according to the specific DNA sequence of the Phytophthora Molecular biology testing.

5 instruments

5.1 PCR instrument. temperature range 4 ° C ~ 99 ° C, temperature control accuracy ± 0.25 ° C. 5.2 Real-time fluorescence PCR instrument. temperature range 4 ° C ~ 99 ° C, temperature control accuracy ± 0.25 ° C. 5.3 Electrophoresis instrument. The output voltage is 5V~600V, and the output current is 2mA~200mA. 5.4 Hybrid furnace. The temperature range is from room temperature to 99.9 °C, and the temperature control accuracy is ±0.1 °C. 5.5 Gel Imaging Analyzer. Continuously adjustable 302nm UV Transilluminator, overhead white light source, white light conversion board, UV interference filter Film, onboard avatar capture software, image analysis software. 5.6 Microscope. Eyepiece magnification factor of 10, objective lens magnification of 100. 5.7 High-speed refrigerated centrifuge. maximum speed 15000r/min. 5.8 Constant temperature incubator. temperature range 5 ° C ~ 65 ° C, fluctuation ± 0.5 ° C. 5.9 Ultra-clean workbench. High-efficiency filter removes more than 99.99% of 0.3μL particles. ......

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