GB/T 36812-2018 English PDF

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GB/T 36812-2018: Molecular detection method of Potato yellow dwarf virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36812-2018	279	Add to Cart	3 days	Molecular detection method of Potato yellow dwarf virus	Valid

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Basic data

Standard ID: GB/T 36812-2018 (GB/T36812-2018)
Description (Translated English): Molecular detection method of Potato yellow dwarf virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,171
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36812-2018: Molecular detection method of Potato yellow dwarf virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular detection method of Potato yellow dwarf virus ICS 65.020.01 B16 National Standards of People's Republic of China Molecular biological detection method of potato yellow dwarf virus Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fuqing Qinghai Entry-Exit Inspection and Quarantine Bureau, Fujian Agriculture and Forestry University, China Academy of Inspection and Quarantine. The main drafters of this standard. Shen Jianguo, Cai Wei, Gao Fangzhen, Zhang Yongjiang, Chen Xihong, Li Min, Wang Yu, Wu Zujian, Chen Shouling. Molecular biological detection method of potato yellow dwarf virus

1 Scope

This standard specifies the molecular biological detection method of potato yellow dwarf virus (Potatoyellowdwarfvirus). This standard applies to quarantine and identification of plants and plant products that may carry potato yellow dwarf virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products SN/T 2964 Plant Virus Testing Practice SN/T 3457 Plant Virus Molecular Biology Testing Specification

3 potato yellow dwarf virus basic information

Chinese name. Potato yellow dwarf virus Scientific name. Potatoyellowdwarfvirus, abbreviation. PYDV. Classification status. Rhabdoviridae, Nucleorhabdovirus. See Appendix A for additional information on potato yellow dwarf virus.

4 Principle of the method

The molecular biological characteristics of potato yellow dwarf virus are the main basis for the development of this test method. 5 instruments, utensils and reagents 5.1 Instrument High-speed refrigerated centrifuge, electronic balance, PCR instrument, fluorescence PCR instrument, horizontal electrophoresis system, -20 ° C low temperature refrigerator and -80 ° C ultra low Warm refrigerator, gel imaging analysis system, pH meter, microwave oven, magnetic stirrer, constant temperature water bath, autoclave, ultra-clean workbench, etc. 5.2 Appliances Adjustable pipette (2.5μL, 10μL, 20μL, 100μL,.200μL, 1000μL) and adjustable pipette tip, centrifuge tube, grinding Hey, etc. 5.3 Reagents Unless otherwise specified, all reagents are of analytical grade, and the experimental water should meet the relevant provisions of GB/T 6682. RT-PCR test See Appendix B for reagents; see Appendix C for real-time fluorescent RT-PCR detection reagents. ......

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