GB/T 36810-2018 English PDF

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GB/T 36810-2018: Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36810-2018	279	Add to Cart	3 days	Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams	Valid

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Basic data

Standard ID: GB/T 36810-2018 (GB/T36810-2018)
Description (Translated English): Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,169
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36810-2018: Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Fusarium oxysporum (Schlecht.) f.sp.Fragariae Winks ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of strawberry wilt pathogen DetectionandidentificationofFusariumoxysporum (Schlecht.)f.sp. FragariaeWinks Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liu Yueting, Luo Jiafeng, Niu Chunjing, Cui Tiejun, Liao Fang. Method for quarantine and identification of strawberry wilt pathogen

1 Scope

This standard specifies the quarantine and identification methods for strawberry wilt pathogens. This standard applies to the quarantine and identification of strawberry wilt pathogens in strawberry seedlings and their carrying soil.

2 Basic information on strawberry wilt pathogen

Chinese name. Strawberry Fusarium Scientific name. Fusariumoxysporum (Schlecht.) f.sp.fragariaeWinks English name. fusariumwiltofstrawberry Classification status. Fungus, Fusarium, Ascomycota, Pezizomycotina, Sordariomycetes, Hypocreales, Nectriaceae, Fusarium. Route of transmission. The germs rely on the seedlings and soil for long-distance transmission. See Appendix A for additional information on strawberry Fusarium.

3 Principle of the method

Symptoms, isolation and culture traits, morphological characteristics and PCR-specific amplified fragments of pathogenic bacteria as quarantine for strawberry wilt Determine the basis.

4 instruments and main reagents

4.1 Apparatus Microscope, ultra-clean workbench, biological incubator, electronic balance, autoclave, regular refrigerator (-20 °C). PCR amplification instrument, electrophoresis Instrument, gel imager, high speed refrigerated centrifuge, constant temperature water bath. Petri dishes, flasks, tweezers, surgical scissors, scalpels, inoculation needles, pipettes, alcohol lamps. 4.2 Primary reagents Ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), Tris-HCl, isoamyl alcohol, chloroform, absolute ethanol, 70% B Molecular biological reagents such as alcohol, potassium chloride, magnesium chloride, proteinase K, primers FofraF/FofraR, Taq polymerase. Or use plant tissue and The fungal genome extraction kit extracts DNA. Sucrose, glucose, agar, penicillin, streptomycin, 0.5% sodium hypochlorite (NaClO). See Appendix B for the preparation of potato sucrose medium (PSA) and potato dextrose medium (PDA).

5 Identification of pathogens

5.1 symptom check Take the plant to be observed, observe whether the plant has poor growth, short stupidity and rot, and whether the lower leaves become purplish red, whether the heart leaves ......

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