GB/T 36789-2018 English PDF

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GB/T 36789-2018: Nucleic acid detection of animal rabies virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36789-2018	279	Add to Cart	3 days	Nucleic acid detection of animal rabies virus	Valid

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Basic data

Standard ID: GB/T 36789-2018 (GB/T36789-2018)
Description (Translated English): Nucleic acid detection of animal rabies virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,123
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36789-2018: Nucleic acid detection of animal rabies virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Animal rabies virus nucleic acid detection method) ICS 11.220 B41 National Standards of People's Republic of China Animal rabies virus nucleic acid detection method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Institute of Military Veterinary Medicine, Academy of Military Medical Sciences. The main drafters of this standard. Feng Wei, Guo Huancheng, Xu Yunbin, Jiang Yan, Tu Changchun.

Introduction

The issuing organization of this document draws attention to the fact that, when the statement conforms to this document, it may involve 4.4 specific aspects related to the nested RT-PCR detection method. Use of Lee. The issuing organization of this document has no position on the authenticity, validity and scope of the patent. The holder of the patent has assured the issuing authority of this document that he is willing to work with any applicant on reasonable and non-discriminatory terms and conditions. Negotiate a patent license. The patent holder's statement has been filed with the issuing authority of this document. Related information can be passed the following Contact information obtained. Name of patent holder. Jiang Yan, Tu Changchun Address. Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, No. 666 Liuyu West Road, Jingyue Economic Development Zone, Changchun City, Jilin Province Please note that in addition to the above patents, certain aspects of this document may still involve patents. The issuing organization of this document does not undertake to identify these special Liability. Animal rabies virus nucleic acid detection method

1 Scope

This standard specifies nested RT-PCR and fluorescence quantitative RT-PCR methods for the detection of animal rabies virus nucleic acids. This standard is applicable to brain tissue, spinal cord, cerebrospinal fluid, salivary gland, saliva and rabies virus in clinically suspected rabies virus-infected animals. Detection of viral nucleic acids in cell culture (see Appendix A).

2 Abbreviations

The following abbreviations apply to this document. RT-PCR. Reverse-Transcription Polymerase Chain Reaction (Reverse-Transcription Polymerase Chain Reaction) DNA. Deoxyribonucleic Acid RNA. Ribonucleic Acid DEPC. Diethypyocarbonate PBS. Phosphate Buffer Solution Ct value. the number of cycles that reach the threshold (CycleThreshold) EDTA. Ethylene Diaminetetraacetic Acid dNTP. Deoxy-RibonucleosideTriphosphate Taq enzyme. Thermos DNA polymerase (ThermusAquaticusPolymerase)

3 equipment and reagents

3.1 Equipment 3.1.1 Instruments Class II biosafety cabinet, 4°C centrifuge, refrigerator (-20°C), freezer (4°C), analytical balance, PCR instrument, real-time PCR Amplifier, electrophoresis, electrophoresis tank, UV gel imager (or UV analyzer), microwave oven, oscillating mixer, tissue grinder, single channel adjustable Pipette (10 μL,.200 μL, 1000 μL). 3.1.2 Consumables Surgical scissors, medical tweezers, weighing paper, cotton swabs, agarose, measuring cylinders (500mL), conical flasks (500mL), disposable RNase-free tips (10μL,.200μL, 1000μL), RNase-free centrifuge tube (1.5mL), PCR tube matched with real-time PCR amplification instrument (0.2 mL), tissue grinding rod. 3.2 Reagents 3.2.1 Total RNA extraction reagents. 3.2.2 Phosphate buffer (1 mol/L PBS, pH 7.4). See Appendix B for the preparation method. 3.2.3 TAE running buffer. See Appendix B for the preparation method. 3.2.4 Other reagents..200 IU/μL reverse transcriptase, 10× reverse transcriptase buffer, 40 U/μL RNase inhibitor, 5IUTaq enzyme, ......

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