GB/T 36779-2018 English PDF

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GB/T 36779-2018: Detection and identification of Fusarium oxysporum f. sp. apii
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Basic data

Standard ID: GB/T 36779-2018 (GB/T36779-2018)
Description (Translated English): Detection and identification of Fusarium oxysporum f. sp. apii
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,190
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36779-2018: Detection and identification of Fusarium oxysporum f. sp. apii

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Fusarium oxysporum f.sp.apii ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of celery wilt pathogen Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang University, Yantai, People's Republic of China Inspection and Quarantine Bureau. The main drafters of this standard. Zhang Weidong, Huang Yonghui, Quan Yongbing, Song Fengming, Chi Yuanli, Xu Weifeng, Liao Li, Lin Wei, Li Jinqing, Yu Jindi. Method for quarantine and identification of celery wilt pathogen

1 Scope

This standard specifies the method for quarantine and identification of celery wilt. This standard is applicable to the quarantine and identification of seeds, seedlings, plant residues and celery wilt pathogens that may carry celery fusarium.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products SN/T 2589 Plant Pathogenic Fungus Detection Specification

3 celery wilt pathogen information

Chinese name. celery wilt pathogen Scientific name. FusariumoxysporumSchlecht.f.sp.apiiSnyderetHansen.1940 Synonym. FusariumbulbigenumCookeetMassee.1887, FusariumorthocerasAppeletWolenw.1910, FusariumangustumSherb.1915, Fusariumorthocerasvar.apii (NelsonetSherb.) WolenwetRein- King.1935, Fusariumapii NelsonetSherb.1937, Fusariumapiivar.pallidum NelsonetSherb.1937, Fusariumoxysporumf.apii (NelsonetSherb.) SnyderetHansen.1940, Fusariumbulbigenum var. Apii (Nelson Classification status. KingdomFungi, Deuteromycota, Hyphomycetes, Neurospora Tuberculariales, Tuberculariaceae, Fusarium. See Appendix A for additional information on celery wilt.

4 Principle

The symptoms of the celery wilt pathogen, the culture characteristics of the colony, the host range, the molecular biological characteristics, and the pathogenicity measurement results are The main basis for its identification. 5 instruments, utensils and main reagents 5.1 Instrument PCR instrument, light incubator, biological microscope (100×~1000×), stereo microscope, tissue grinder, biological safety cabinet, high speed cold Freeze centrifuge, micro spectrophotometer, electrophoresis instrument, gel imaging system, electronic balance (inductance 0.0001g), pH meter, autoclave. 5.2 Appliances Alcohol lamps, gauze, beakers, inoculation needles, inoculating rings, petri dishes, conical flasks, cotton plugs, slides, coverslips, aluminum platinum paper, scalpels, anatomy ......

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