GB/T 36760-2018 English PDF

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GB/T 36760-2018: Term and classification of reagent enzymes
Status: Valid

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GB/T 36760-2018	199	Add to Cart	3 days	Term and classification of reagent enzymes	Valid

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Basic data

Standard ID: GB/T 36760-2018 (GB/T36760-2018)
Description (Translated English): Term and classification of reagent enzymes
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 10,112
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36760-2018: Term and classification of reagent enzymes

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Term and classification of reagent enzymes ICS 07.080 C27 National Standards of People's Republic of China Tool enzyme terminology and classification Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Tool Enzyme Standardization Working Group (SAC/SWG11). This standard was drafted. Fujian Huacan Pharmaceutical Co., Ltd., Fujian Nansheng Technology Co., Ltd., Fudan University, Shandong University, Angel Yeast Co., Ltd., Institute of Metrology and Analysis, China Institute of Metrology, Beijing University of Chemical Technology, Fujian Agriculture and Forestry University, China Agricultural University Xue, Suzhou Beaver Biomedical Engineering Co., Ltd., Shanghai Bo Shi Biomedical Service Center, Suzhou University, Chinese Academy of Sciences, Microbiology Research Institute, Xiamen Zhishan Biotechnology Co., Ltd., Anhui University, South China University of Technology, Shanghai Baisai Biotechnology Co., Ltd. The main drafters of this standard. Huang Facan, Zheng Dengzhong, Zhan Xuexiong, Zhao Jing, Zhong Jiang, Chen Xiulan, Yao Wei, Zhang Lili, Quan Can, Chen Jinchun, Liu Bin, Li Quanhong, Zhang Xiying, Ren Hui, Xing Zhigang, Zhu Li, Liu Wenjun, Li Jing, Song Najie, Li Qingge, Wang Shihua, Huang Faxi, Xiao Yazhong, Song Daxin, Lin Ying, Liu Pei.

Introduction

The tool enzyme is a system for cleavage, ligation, synthesis (amplification), modification, and the like of biological molecules such as nucleic acids, proteins, sugars, and lipids. It is used in biomedical and life science related fields such as genetic engineering, enzyme engineering, protein engineering, and cell engineering. Tool enzymatic National standards for language, definition and classification to promote the industrialization of tool enzymes are of great significance for the production and use of tool enzymes. Tool enzyme terminology and classification

1 Scope

This standard specifies the terms, definitions and classifications of the tool enzymes. This standard applies to tool enzymes involved in research, production and application processes.

2 Terms and definitions

2.1 Tool enzyme reagentenzymes A general term for reagent enzymes such as cleavage, ligation, synthesis (amplification), and modification of biological molecules such as nucleic acids, proteins, sugars, and lipids. Note. Applied to biomedical and life science related fields such as genetic engineering, enzymatic engineering, protein engineering, and cell engineering. 2.2 Restriction endonuclease restrictioniononuclease Having a phosphodiester bond that identifiable to a specific nucleotide sequence and between two nucleotides at a specific part of each strand The enzyme of action. 2.3 Endonuclease An enzyme having the function of catalyzing the hydrolysis of a 3,5-phosphodiester bond from the middle of a polynucleotide chain to release a short fragment of an oligonucleotide. 2.4 Exonuclease exonuclease It has the ability to sequentially hydrolyze 3,5-diphosphate bond from one end of the polynucleotide chain and degrade into a single nucleotide (DNA is dNMP, an enzyme in which RNA acts as NMP). Note. DNA (full name Deoxyribonucleicacid) is deoxyribonucleic acid; RNA (full name Ribonucleic acid) is ribonucleic acid. 2.4.1 Single-stranded exonuclease single-strandedexonuclease An enzyme having the function of catalyzing the degradation of DNA from a 5'-end or a 3'-end of a single-stranded nucleic acid, releasing a short fragment of the oligonucleotide. 2.4.2 Double-stranded exonuclease double-strandedexonuclease An enzyme having the action of catalyzing the release of a 5'-mononucleotide from the 3'-OH end in a 3'- >5' direction of double-stranded DNA. 2.5 Polymerase polymerase It has an enzyme capable of catalyzing the action of synthesizing a new nucleic acid strand using a nucleic acid strand as a template. Includes DNA polymerase and RNA polymerase. 2.5.1 DNA polymerase DNA polymerase It has an enzyme that catalyzes the action of synthesizing a new DNA strand using a DNA strand as a template. 2.5.2 RNA polymerase RNA polymerase It has an enzyme that catalyzes the action of synthesizing a new RNA strand using a DNA or RNA strand as a template. ......

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