GB/T 35918-2018 English PDFUS$359.00 ยท In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35918-2018: Identification of animal origin in animal products by DNA barcoding -- Sanger sequencing Status: Valid
Basic dataStandard ID: GB/T 35918-2018 (GB/T35918-2018)Description (Translated English): Identification of animal origin in animal products by DNA barcoding -- Sanger sequencing Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A40 Classification of International Standard: 07.080 Word Count Estimation: 18,144 Date of Issue: 2018-02-06 Date of Implementation: 2018-09-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 35918-2018: Identification of animal origin in animal products by DNA barcoding -- Sanger sequencing---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Identification of animal origin in animal products by DNA barcoding - Sanger sequencing ICS 07.080 A40 National Standards of People's Republic of China Animal - derived products in the detection of gene barcode technology Sanger sequencing 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard was drafted. China Academy of Inspection and Quarantine. The main drafters of this standard. Wu Yajun, Chen Ying, Yang Yan Song, Huang Wensheng, Wang Bin, Han Jianxun, Liu Ming Chang, Zhang Jikai, Deng Tingting. Animal - derived products in the detection of gene barcode technology Sanger sequencing1 ScopeThis standard specifies the gene barcode technology Sanger sequencing principle, the main reagents, the main equipment, sample collection and preparation, seized Check the steps, quality control, the results of judgment and expression, testing to prevent cross-infection measures. This standard applies to mammals, birds, fish animals, meat, milk, blood, hair, angle bones, viscera, skin and other sources of animal products and Qualitative detection of feed species. Note 1. Refer to informative Annex A for species. Note 2. Gene barcode Sanger sequencing method detection limit of 10% (mass fraction).2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods GB/T 9695.19 meat and meat products sampling method GB/T 14699.1 feed sampling Transgenic products - Detection of nucleic acid extraction and purification methods GB/T 19495.3-2004 GB/T 21104 animal-derived feed ruminant-derived components (cattle, sheep, deer) qualitative detection methods PCR method Laboratory quality control specifications - Food molecular biology tests SN/T 3500-2013 Import and export food safety biological testing sampling specification 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Gene barcode DNAbarcode An accepted, readily mutable, and relatively short DNA fragment of the organism that is capable of representing the standard of the species. 3.1.2 Sanger Sequencing Sangersequencing Dideoxy chain termination method Using single-stranded or double-stranded DNA as a template, a primer that binds to the template is extended under the catalysis of a DNA polymerase until a chain is incorporated Stop the nucleotides. Each reaction contained four dNTPs, mixed with isotope-labeled or fluorescently labeled ddNTPs, allowing the extended oligonucleotidytes The selective termination of the nucleotide sequence and the isolation of the fragments by high-resolution denaturing capillary electrophoresis result in a base sequence. 3.1.3 Gene barcode technology DNAbarcoding Using gene sequencing technology to identify the species of gene barcode, using its species specificity and species diversity to achieve the species fast ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35918-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 35918-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35918-2018_English with my colleagues?Answer: Yes. 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