GB/T 35912-2018 English PDF

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GB/T 35912-2018: Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35912-2018	199	Add to Cart	3 days	Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus	Valid

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Basic data

Standard ID: GB/T 35912-2018 (GB/T35912-2018)
Description (Translated English): Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,185
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35912-2018: Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus ICS 11.220 B41 National Standards of People's Republic of China Porcine reproductive and respiratory syndrome virus Fluorescent RT-PCR detection method Published on.2018-02-06 2018-09-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture of the People's This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, China People's Republic of China Shenzhen Entry-Exit Inspection and Quarantine Bureau, Hunan Shengxiang Bio-Technology Co., Ltd., and China Academy of Inspection and Quarantine. The main drafters of this standard. Zhang Qiang, Li Jian, Xiong Wei, Tong Guangzhi, Zhao Heping, Li Shuqing, Hua Qunyi, Yang Zhongping, Li Guoxin, Wang Qiaoquan, Wang Yan, Lin Yingkai, Cai Kaimei, Yu Zhengjun, Lin Xiangmei, and Wu Shaoqiang. Porcine reproductive and respiratory syndrome virus Fluorescent RT-PCR detection method

1 Scope

This standard specifies the operation method for the detection of porcine reproductive and respiratory syndrome virus fluorescence RT-PCR. This standard applies to the nucleic acid detection of the porcine reproductive and respiratory syndrome virus American strain.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 25172 Technical Specification for Production and Preservation of Semen at Normal Temperature in Pigs

3 Abbreviations

The following abbreviations apply to this document. Real-time RT-PCR. Real-time reverse transcription polymerase chainre- Action) Ct value. the number of cycles experienced when the amount of fluorescence signal in each reaction tube reaches the set threshold value (cyclethreshold) RNA. ribonucleic acid Taq enzyme. Taq DNA polymerase TE buffer. Tris-EDTA buffer DEPC. diethylpyrocarbonate PRRSV. Porcine reproductive and respiratory syndrome virus (porcinereproductiveandrespiratorysyndromevirus) PBS. phosphate buffered solution

4 Instruments

4.1 Fluorescent PCR detector. 4.2 High-speed desktop refrigerated centrifuge. Controllable temperature up to 4 °C, centrifugal speed up to 12000r/min or more. 4.3 Tissue grinder or mortar. 4.4 ordinary refrigerator. 2 °C ~ 8 °C. 4.5 ultra-low temperature refrigerator. Controllable temperature to -70 °C below. 4.6 Micropipettes. 0.2μL~2μL, 1μL~10μL, 10μL~100μL, 20μL~200μL, 100μL~1000μL, and Equipped with a pipette to match the tip. 4.7 Autoclave. ......

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