GB/T 35911-2018 English PDFUS$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35911-2018: Real-time PCR method for detection of pseudorabies virus Status: Valid
Basic dataStandard ID: GB/T 35911-2018 (GB/T35911-2018)Description (Translated English): Real-time PCR method for detection of pseudorabies virus Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 10,136 Date of Issue: 2018-02-06 Date of Implementation: 2018-09-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 35911-2018: Real-time PCR method for detection of pseudorabies virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Real-time PCR method for detection of pseudorabies virus ICS 11.220 B41 National Standards of People's Republic of China Pseudorabies virus fluorescent PCR detection method Published on.2018-02-06 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture of the People's Republic This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, China Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine, Hunan Shengxiang Biotechnology Co., Ltd. The main drafters of this standard. Zhang Qiang, Tong Guangzhi, Li Jian, Xiong Wei, Zhao Heping, Li Shuqing, Wang Yan, Li Guoxin, Yang Zhongping, Hua Qunyi, Lin Yingxi, Wang Qiaoquan, Cai Kaimei, Yu Zhengjun, Wu Shaoqiang, Lin Xiangmei. Pseudorabies virus fluorescent PCR detection method1 ScopeThis standard specifies the method for the detection of pseudorabies virus fluorescent PCR. This standard applies to the detection of pseudorabies virus nucleic acid.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 25172 Pig normal temperature semen production and preservation technical specifications3 AbbreviationsThe following abbreviations apply to this document. Fluorescent PCR fluorescent polymerase chain reaction (real-time polymerase chain reaction) Ct value Cycle number (cyclethreshold) experienced when the amount of fluorescent signal in each reaction tube reaches a set threshold DNA deoxyribonucleic acid (deoxyribonucleic acid) Taq enzyme Taq DNA polymerase (TaqDNApolymerase) TE buffer Tris-EDTA buffer (Tris-EDTAbuffer) PRV pseudorabies virus (pseudorabiesvirus)4 instruments4.1 Fluorescence PCR detector. 4.2 High-speed desktop refrigerated centrifuge. can control temperature to 4 ° C, centrifugal speed can reach 12000r/min or more. 4.3 Tissue grinder or mortar. 4.4 Ordinary refrigerator. 2 ° C ~ 8 ° C. 4.5 Ordinary freezer. below -20 °C. 4.6 Ultra-low temperature refrigerator. can control temperature below -70 °C. 4.7 Micropipette. 0.2μL~2μL, 1μL~10μL, 10μL~100μL, 20μL~200μL, 100μL~1000μL, and Comes with a tip that matches the pipette. 4.8 Autoclave.5 supplies5.1 1.5 mL centrifuge tube. 5.2 0.2mL PCR thin wall tube or eight tube. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35911-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 35911-2018_English as soon as possible, and keep you informed of the progress. 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