GB/T 35909-2018 English PDF

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GB/T 35909-2018: Detection of mycoplasma hyopneumoniae using PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35909-2018	199	Add to Cart	3 days	Detection of mycoplasma hyopneumoniae using PCR method	Valid

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Basic data

Standard ID: GB/T 35909-2018 (GB/T35909-2018)
Description (Translated English): Detection of mycoplasma hyopneumoniae using PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,112
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35909-2018: Detection of mycoplasma hyopneumoniae using PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of mycoplasma hyopneumoniae using PCR method ICS 11.220 B41 National Standards of People's Republic of China Mycoplasma hyopneumoniae PCR detection method 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted. Jiangsu Academy of Agricultural Sciences. Drafters of this standard. Shao Guoqing, Feng Zhixin, Xiong Qi Yan, Liu Maojun, Zhang Lei, Wang Haiyan, Wu Yuzi, Wei Yanna, Liu Beibei, Wang Jia, Wang Li. Mycoplasma hyopneumoniae PCR detection method

1 Scope

This standard specifies the method of PCR detection of Mycoplasma hyopneumoniae. This standard applies to laboratory clinical samples of pigs (swine nasal swabs, lung and bronchoalveolar lavage fluid), bacterial culture and animal production Rapid detection of Mycoplasma hyopneumoniae nucleic acid in

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods GB 19489 laboratory biosafety common requirements GB/T 27401 Laboratory Quality Control Animal Quarantine Diseases and disease harmless animal handling technical specifications Nongyifa [2017] No. 25 Veterinary Laboratory Biosafety Regulations (People's Republic of China Ministry of Agriculture Bulletin No. 302)

3 Terms and definitions and abbreviations

3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Mycoplasma hyopneumoniae; MHP Mycoplasma Mycoplasma is a member of the Mycoplasma pneumoniae (MPS) major pathogens. Note. Mycoplasma hominis also known as swine asthma, is a chronic infection of the respiratory tract, causing serious damage to the pig industry. 3.2 Abbreviations The following abbreviations apply to this document. PCR polymerase chain reaction (polymerasechainreaction) PBS phosphate buffer (phosphate-bufferedsalinebuffer) TAE TAE electrophoresis buffer (tris-acetate-ethylenediaminetetraacetic acid buffer) TBE TBE electrophoresis buffer (tris-borate-ethylenediaminetetraacetic acid buffer) Taq polymerase Taq DNA polymerase (Taq DNA polymerase) dNTPs deoxyribonucleosidetriphosphates DNA deoxyribonucleic acid CCU color change unit (colorchangeunit) MPS Mycoplasma pneumonia of swine ......

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