GB/T 35539-2017 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35539-2017: Restriction endonuclease BamH Ⅰ Status: Valid
Basic dataStandard ID: GB/T 35539-2017 (GB/T35539-2017)Description (Translated English): Restriction endonuclease BamH �� Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C27 Classification of International Standard: 07.080 Word Count Estimation: 14,169 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35539-2017: Restriction endonuclease BamH Ⅰ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Restriction endonuclease BamHⅠ ICS 07.080 C27 National Standards of People's Republic of China Restriction endonuclease BamHI 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Foreword Ⅲ Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 technical requirements5 test methods 1 6 packaging, transportation and storage 2 7 shelf life 2 Appendix A (Normative) Restriction endonuclease BamH Ⅰ enzyme activity test 3 Appendix B (Normative) Other endonuclease detection 6 Appendix C (Normative) Exonuclease assay 7ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized. This standard drafting unit. Beijing University of Chemical Technology, Fujian Nansheng Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., China Metrology Institute, Hill East University, Sanming City Quality and Technical Supervision Association, Angel Yeast Co., Ltd., Fujian Agriculture and Forestry University. The main drafters of this standard. Chen Jinchun, Chen Gang, Wang Liren, Huangfa Can, Quan Can, Zheng Dengzhong, Zhan Xuexiong, Zhuang Liqiong, Zheng Qiao Rong, Huang Fa, Zhao Jing, Chen Xiulan, Yao Juan, Liu Bin.IntroductionBamH I is a restriction endonuclease that specifically recognizes the G ↓ GATCC base sequence in the DNA strand and produces 5 ' End. Formulation of BamH I national standards, to promote the industrialization of such tools, enzymes, for the production and use of BamH I have important significance. Restriction endonuclease BamHI1 ScopeThis standard specifies the technical requirements of restriction endonuclease BamH Ⅰ, test methods, packaging, transport, storage and shelf life. This standard applies to bacterial BacilusamyloliquefaciensH fermentation preparation or genetically engineered bacteria Escherichia.coli Of BamHI.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 Packaging - Pictorial signs GB/T 6682 analytical laboratory water specifications and experimental methods3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 BamH I A restriction endonuclease that specifically recognizes the base sequence on the DNA strand G ↓ GATCC produces 5 'sticky ends derived from Bacilus amyloliquefaciens H or Bacilus subtilis MT-2 (pBamHI RM22) or a recombinant E. coli strain containing BamHI gene cloned from Bacilus amyloliquefaciens H strain. 3.2 BamHI activity unit BamH I activityunit The amount of enzyme required to completely decompose 1 μg of λDNA was 1 viable unit (1 U) at 37 ° C for 1 h in a 50 μL reaction.4 technical requirements4.1 appearance Clear and transparent liquid, no sediment. 4.2 enzyme activity ≥10000U/mL. 4.3 Impurities It should not contain other endonucleases and exonucleases.5 test methods5.1 appearance Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35539-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35539-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35539-2017_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 35539-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |
