GB/T 35541-2017 English PDF

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GB/T 35541-2017: pfu DNA polymerase
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35541-2017	279	Add to Cart	3 days	pfu DNA polymerase	Valid

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Basic data

Standard ID: GB/T 35541-2017 (GB/T35541-2017)
Description (Translated English): pfu DNA polymerase
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Classification of International Standard: 07.080
Word Count Estimation: 14,150
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35541-2017: pfu DNA polymerase

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
pfu DNA polymerase ICS 07.080 C27 National Standards of People's Republic of China pfu DNA polymerase 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Foreword Ⅲ Introduction IV 1 Scope 1 2 Normative references 1 3 Terms and definitions 1

4 technical requirements

5 test method 2 6 packaging, transportation and storage 2 7 shelf life 2 Appendix A (Normative) pfuDNA polymerase enzyme activity test 3 Appendix B (Normative) Exonuclease assay 6 Appendix C (Normative) Endonuclease assay 8

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Tooling Standardization Working Group (SAC/SWG11) centralized. This standard was drafted. Xiamen Zhi Shan Biological Technology Co., Ltd., Fujian Hua Can Pharmaceutical Co., Ltd., Fujian South Health Technology Co., Ltd. Division, Shenzhen Huaneng Kang Gene Technology Co., Ltd., Huacan Nansheng (Xiamen) Biotechnology Co., Ltd., Institute of Microbiology, Chinese Academy of Sciences, Xiamen University, Beijing University of Chemical Technology, Shandong University, Fudan University, Beaver Nano Technology (Suzhou) Co., Ltd., Shanghai Boshi Biological Technology Co., Ltd., Angel Yeast Co., Ltd., China Agricultural University, Fujian Agriculture and Forestry University, Shanghai 100 赛 Biological Technology Co., Ltd. The main drafters of this standard. Song Najie, Huangfa Can, Zheng Dengzhong, He Changhua, Zhan Xuexiong, Li Qing Court, Zhao Jing, Li Quanhong, Sheng Tong, Chen Jinchun, Chen Xiulan, Zhong Jiang, Liu Bin, Yao Juan, Ren Hui, Xing Zhigang, Huang Fa, Zhang Xying, Teresa Teng, Zhang Lili.

Introduction

The pfuDNA polymerase is derived from Pyrococcus fariosus and has a very high thermostability and fidelity and is widely applied For routine molecular biology experiments. Development of pfu DNA polymerase national standards to promote the industrialization of such tools, enzymes, pfu DNA polymerase production and use of great significance. pfu DNA polymerase

1 Scope

This standard specifies the technical requirements pfuDNA polymerase, test methods, packaging, transportation, storage and shelf life. This standard applies to genetically engineered bacteria made of pfuDNA polymerase.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 191 Packaging - Pictorial signs.

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 One from Pyrococcus furiosus, molecular weight of 90ku, with high thermal stability and fidelity DNA polymerase. This enzyme catalyzes the polymerization of deoxyribonucleotides onto the primers from the 5'-3 'direction of the template, with 3'-5' exonuclease activity, none 5'-3 'exonuclease activity. 3.2 Using a synthetic hairpin-type oligonucleotide sequence as a template/primer, 0.14 nmol of deoxynucleotide was polymerized into The amount of enzyme required in double stranded DNA is 1 viable unit (U).

4 technical requirements

4.1 appearance Clear transparent liquid, no sediment. 4.2 enzyme activity ≥5000U/mL. 4.3 Impurities It should not contain exonucleases and endonucleases. ......

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