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GB/T 35338-2017 English PDF

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GB/T 35338-2017: Detection and identification of Cadophora gregata (Allington & D. W. Chamb.) T. C. Harr. & McNew
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 35338-2017279 Add to Cart 3 days Detection and identification of Cadophora gregata (Allington & D. W. Chamb.) T. C. Harr. & McNew Valid

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Basic data

Standard ID: GB/T 35338-2017 (GB/T35338-2017)
Description (Translated English): Detection and identification of Cadophora gregata (Allington & D. W. Chamb.) T. C. Harr. & McNew
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,171
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35338-2017: Detection and identification of Cadophora gregata (Allington & D. W. Chamb.) T. C. Harr. & McNew


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cadophora gregata (Allington ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of soybean brown stem rot Published on.2017-12-29 2018-07-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Commission for Standardization of Plant Quarantine (SAC/TC271). This standard was drafted by. Shenzhen Institute of Inspection and Quarantine, People's Republic of China Shenzhen Entry-Exit Inspection and Quarantine Bureau, Chinese People Fujian Entry-Exit Inspection and Quarantine Bureau, China Academy of Inspection and Quarantine. The main drafters of this standard. Chen Zhinan, Wang Nianwu, Du Hongzhong, Wang Ying, Wu Pinshan, Cheng Yinghui, Wang Ying, Zhang Guiming, Li Fangrong. Method for quarantine and identification of brown stem rot of soybean stem

1 Scope

This standard specifies the quarantine and identification methods for soybean brown stem rot. This standard is applicable to the quarantine and identification of brown rot pathogens of soybean stem stalks caused by pathogens of soybean brown rot.

2 basic information of germs

Scientific name. Cadophoragregata (Alington Synonyms. Cephalosporium gregatum Alington DWChamb.) TCHarr. Taxonomic status. Fungi, Ascomycota, Eurotiomycetes, Chae- Tothyriales), Herpotrichielaceae. There are 2 host specialization types of soybean brown stem rot pathogen, namely soybean stem brown rot pathogen soybean specialized type C. gregataf.sp.sojae and soybean There are no obvious differences in morphological and physiological characteristics between the two species, C. gregataf.sp.adzukicola. Difference between DNA sequence and host, G. capsularis infection with red beans does not infect soybeans, and soybean brown stem rot does not infect soybeans with brown rot. beans. According to the differences in DNA sequence and symptoms, the soybean-species soy cultivar C. gregataf.sp.sojae was divided into two groups. Genotypes, type A and type B. Route of transmission. Brown stem rot of soybean stems spreads mainly through the host plant stem residues. See Appendix A for additional information on soybean brown rot fungi and Appendix B for sequence information.

3 Methodology

According to the culture traits, morphological characteristics and molecular biology characteristics of the brown stem rot of soybean stems as the judgement basis.

4 Instruments and apparatus

4.1 Instruments The main instruments used include. biological microscopes, clean benches, light incubators, electronic balances (1/1000g), autoclaves, Refrigerator, PCR, electrophoresis, gel imager, high-speed refrigerated centrifuge, constant temperature water bath, shaker. 4.2 Appliances The main appliances used include. beakers, flasks, graduated cylinders, test tubes, petri dishes, alcohol lamps, tweezers, scissors, scalpels, inoculation needles, glass carriers Tablets, coverslips, plastic mortar, pipettes, pipette tips, centrifuge tubes, liquid nitrogen tanks.

5 Reagents and culture media

5.1 Reagents Unless otherwise specified, all reagents were of analytical grade.
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