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GB/T 35330-2017 English PDF

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GB/T 35330-2017: Detection and identification of Hosta virus X
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 35330-2017359 Add to Cart 4 days Detection and identification of Hosta virus X Valid

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Basic data

Standard ID: GB/T 35330-2017 (GB/T35330-2017)
Description (Translated English): Detection and identification of Hosta virus X
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 18,122
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Regulation (derived from): National Standards Bulletin 2017 No. 32
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35330-2017: Detection and identification of Hosta virus X

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Hosta virus X ICS 65.020.01 B16 National Standards of People's Republic of China Hosta genus X virus quarantine and identification methods 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Committee for Phytosantry Standardization Technical Committee (SAC/TC271). This standard drafting unit. China Inspection and Quarantine Science Institute, the State Administration of Quality Supervision, Inspection and Quarantine Information Center, People's Republic of China Exit Inspection and Quarantine of Xiamen, Exit Inspection and Quarantine of People's Republic of China, Exit Inspection and Quarantine of People's Republic of China The epidemic, China Agricultural University. The main drafters of this standard. Zhang Yongjiang, Zhang Yanping, Fang Zhipeng, Xin Yan, Wei Meisheng, Feng Lixia, Shen Jianguo, Li Guifen, Li Man, Zhu Shuifang. Hosta genus X virus quarantine and identification methods

1 Scope

This standard specifies the Hosta virus X (HostavirusX) quarantine and identification methods. This standard applies to the host may carry Hosta X plant and its products, quarantine and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods SN/T 2122 Quarantine and sampling methods for entry and exit of plants and plant products SN/T 2964 plant virus detection specification SN/T 3457 Codex specification for plant virus molecular biology

3 Hosta Genus X virus basic information

Scientific name. HostavirusX. Abbreviation .HVX. Synonym Hosta virus X, Hosta X virus, Hosta X virus, Hosta (Liliaceae) plant virus. Taxonomic status. Flexiviridae, Potexvirus. See Appendix A for additional information on hosta X viruses.

4 method principle

Hosta X-immunogenicity and genomic characteristics of the virus is based on the identification of quarantine. Depending on the HVX and the antibody Specific reaction, the plant samples were double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and colloidal gold immunoassay strip test; RT-PCR, real-time fluorescence RT-PCR and RT-LAMP detection methods were established based on the genomic characteristics of HVX. Effective combination to determine whether the sample with HVX. 5 instruments, appliances and reagents 5.1 Instruments Electronic Balance (Feel 0.001g), high-speed refrigerated centrifuge, small instantaneous centrifuge, general refrigerator, ultra-low temperature refrigerator (-80 ℃), the system Ice machine, vortex shaker, magnetic stirrer, autoclave, pH meter, clean bench, PCR instrument, microwave oven, electrophoresis apparatus, electrophoresis tank, gel Imaging Analyzer, microplate reader. 5.2 appliances Enzyme-linked plate, adjustable pipette (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and adjustable pipette tip, centrifuge tube,
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