GB/T 35337-2017 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35337-2017: Detection and identification of Grapevine yellow speckle viroid Status: Valid
Basic dataStandard ID: GB/T 35337-2017 (GB/T35337-2017)Description (Translated English): Detection and identification of Grapevine yellow speckle viroid Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,199 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35337-2017: Detection and identification of Grapevine yellow speckle viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Grapevine yellow speckle viroid ICS 65.020.01 B16 National Standards of People's Republic of China Grape yellow point virus quarantine and identification methods 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Committee for Phytosantry Standardization Technical Committee (SAC/TC271). This standard drafting unit. China Inspection and Quarantine Science Institute, People's Republic of China Beijing Exit Inspection and Quarantine, China Agricultural Branch Institute of Plant Protection. The main drafters of this standard. Zhang Yongjiang, Deng Congliang, Luo Weifeng, Zhao Xiaoli, Lv Yufeng, Zhang Zhi want, Xin Yan said. Grape yellow point virus quarantine and identification methods1 ScopeThis standard specifies the grape yellow point virus quarantine and identification methods. This standard applies to the grapes may contain yellow-point virus grape reproductive material identification.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods3 grape yellow point virus basic informationScientific name. Grapevineyellowspeckleviroid. Abbreviation .GYSVd. Synonym. Grapevine yellow virus, grape yellow pox virus. Taxonomic status Potato spindle-spiked Pospiviroidae, Apscaviroid. Refer to Appendix A for additional information on grape yellow spot virus.4 method principleViral detection mainly targets its nucleic acid sequence. The main methods are RT-PCR and nucleic acid hybridization. 5 equipment, main appliances and main reagents 5.1 Equipment Electronic balance (flu volume 0.001g), ordinary balance (flu volume 0.1g), high-speed refrigerated centrifuge, small instantaneous centrifuge, general refrigerator, super Low temperature refrigerator (-80 ℃), ice maker, vortex shaker, magnetic stirrer, autoclave, pH meter, PCR instrument, microwave ovens, electrophoresis instrument, electricity Swimming pool, gel analysis and imaging system, real-time fluorescence PCR instrument, hybrid furnace, plastic film sealing machine, UV cross-linking instrument, dark box, shaker, constant temperature water Bath, tissue crusher and so on. 5.2 main appliances Adjustable pipette (2.5 μL, 10 μL, 20 μL,.200 μL, 1000 μL, 5000 μL), RNase-free tip (10 μL, 20 μL, 200 μL, 1000 μL, 5000 μL), RNase-free centrifuge tube (1.5 mL, 5 mL, 10 mL), mortar, pestle, magnetic separator, PCR Reaction tube (200 μL), real-time fluorescence 96-well reaction plate, hybridization flask, hybrid membrane, hybridization bag, X-ray. 5.3 The main reagent Unless otherwise specified, all reagents are of analytical grade, the experimental water should be consistent with the relevant provisions of GB/T 6682. Ordinary RT-PCR test ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35337-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35337-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35337-2017_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 35337-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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