GB/T 35336-2017 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35336-2017: Detection and identification of Apple fruit crinkle viroid Status: Valid
Basic dataStandard ID: GB/T 35336-2017 (GB/T35336-2017)Description (Translated English): Detection and identification of Apple fruit crinkle viroid Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,183 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35336-2017: Detection and identification of Apple fruit crinkle viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Apple fruit crinkle viroid ICS 65.020.01 B16 National Standards of People's Republic of China Apples wrinkle fruit virus quarantine and identification methods 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Committee for Phytosantry Standardization Technical Committee (SAC/TC271). This standard was drafted by Beijing Entry-Exit Inspection and Quarantine Bureau of People's Republic of China, China Academy of Inspection and Quarantine. The main drafters of this standard.Deng Congliang, Zhao Xiaoli, Lv Yufeng, Liang Xinmiao, Luo Weifeng, Zheng Chunsheng, Zhang Yongjiang. Apples wrinkle fruit virus quarantine and identification methods1 ScopeThis standard specifies the method of quarantine and identification of apple fry virus. This standard applies to the detection and identification of plant material which may have apple wrinkle fruit virus.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods3 apple wrinkled fruit virus basic informationScientific name .Applefruitcrinkleviroid. Abbreviation .AFCVd. Taxonomic status Potato spindle-spiked Pospiviroidae, Apscaviroid. See appendix A for additional information on apple-wrinkled fruit viruses.4 method principleViral detection mainly targets its nucleic acid sequence. The main methods are RT-PCR and nucleic acid hybridization. 5 equipment, main appliances and main reagents 5.1 Equipment Electronic balance (flu volume 0.001g), ordinary balance (flu volume 0.1g), high-speed refrigerated centrifuge, small instantaneous centrifuge, general refrigerator, super Low temperature refrigerator (-80 ℃), ice maker, vortex shaker, magnetic stirrer, autoclave, pH meter, PCR instrument, microwave ovens, electrophoresis instrument, electricity Swimming pool, gel analysis and imaging system, real-time fluorescence PCR instrument, hybrid furnace, plastic film sealing machine, UV cross-linking instrument, dark box, shaker, constant temperature water Bath, tissue crusher and so on. 5.2 main appliances Adjustable pipette (2.5 μL, 10 μL, 20 μL,.200 μL, 1000 μL, 5000 μL), RNase-free tip (10 μL, 20 μL, Mu] L, 1000 [mu] L, 5000 [mu] L), RNAse free centrifuge tubes (1.5 mL, 5 mL, 10 mL), mortar, pestle, magnetic separator Reaction tube (200 μL), real-time fluorescence 96-well reaction plate, hybridization flask, hybrid membrane, hybridization bag, X-ray. 5.3 The main reagent Unless otherwise specified, all reagents are of analytical grade, the experimental water should be consistent with the relevant provisions of GB/T 6682. RT-PCR test See Appendix B for real-time fluorescent RT-PCR reagents; Appendix C for dot-blot hybridization assays. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35336-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35336-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35336-2017_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 35336-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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