GB/T 35332-2017 English PDFUS$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 35332-2017: Detection and identification of Grapevine virus A Status: Valid
Basic dataStandard ID: GB/T 35332-2017 (GB/T35332-2017)Description (Translated English): Detection and identification of Grapevine virus A Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,180 Date of Issue: 2017-12-29 Date of Implementation: 2018-07-01 Regulation (derived from): National Standards Bulletin 2017 No. 32 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 35332-2017: Detection and identification of Grapevine virus A---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Grapevine virus A ICS 65.020.01 B16 National Standards of People's Republic of China Grapes A virus quarantine and identification methods 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Committee for Phytosantry Standardization Technical Committee (SAC/TC271). This standard was drafted by China Academy of Inspection and Quarantine, Fujian Academy of Agricultural Sciences, People's Republic of China Exit Inspection Quarantine Bureau, People's Republic of China Exit Inspection and Quarantine of People's Republic of China. The main drafters of this standard. Zhang Yongjiang, Xie Lixue, Li Bin, Wei Meisheng, Li Guifen, Ma Jie, Xin Yan Yan, Zhang Xiaolei, Wu Xinghai, Zhu Shuifang. Grapes A virus quarantine and identification methods1 ScopeThis standard specifies the grape A virus (GrapevinevirusA) quarantine methods. This standard applies to the grapes may carry grapevine seedlings, scions, rootstocks, cuttings and other propagation materials, quarantine and identification.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods SN/T 1840 plant virus immune electron microscopy detection method3 grape A virus basic informationScientific name. GrapevinevirusA. Abbreviation .GVA. Synonyms. Grapevines tempting-associated virus. Taxonomic status. Betaflexiviridae, Vitivirus. See Appendix A for additional GVA information.4 method principleThe protein and genomic characteristics of GVA are the basis of the virus quarantine and identification. According to the specific reaction between GVA and antibody, on the plant The samples were detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunoelectron microscopy; according to the genomic characteristics of GVA RT-PCR, real-time fluorescence RT-PCR; judging by the effective combination of these methods whether the sample has GVA. 5 instruments, appliances and reagents 5.1 Instruments Electronic Balance (Feel 0.001g), high-speed refrigerated centrifuge, small instantaneous centrifuge, general refrigerator, ultra-low temperature refrigerator (-80 ℃), the system Ice machine, vortex shaker, magnetic stirrer, autoclave, pH meter, clean bench, PCR instrument, microwave oven, electrophoresis apparatus, electrophoresis tank, gel Imaging Analyzer, Microplate Reader, Transmission Electron Microscope. 5.2 appliances Enzyme-linked plate, adjustable pipette (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and adjustable pipette tip, centrifuge tube, Bowl, micro-grinding pestle and so on. 5.3 Reagents Unless otherwise specified, all reagents are of analytical grade, the experimental water should be consistent with the relevant provisions of GB/T 6682. DAS-ELISA reagent ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 35332-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 35332-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 35332-2017_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 35332-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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