GB/T 35329-2017 English PDF

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GB/T 35329-2017: Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 35329-2017	279	Add to Cart	3 days	Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell	Valid

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Basic data

Standard ID: GB/T 35329-2017 (GB/T35329-2017)
Description (Translated English): Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,152
Date of Issue: 2017-12-29
Date of Implementation: 2018-07-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35329-2017: Detection and identification of Phytophthora medicaginis E. M. Hans. et D. P. Maxwell

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora medicaginis EMHans.et DPMaxwell ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification methods for root rot pathogens Published on.2017-12-29 2018-07-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Commission for Standardization of Plant Quarantine (SAC/TC271). This standard was drafted by the People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau and the Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Zhang Yujun, Du Hongzhong, Guo Jingze, He Yan, Liu Peng, Liu Yueting, Wu Pinshan. Quarantine and identification methods for root rot pathogens

1 Scope

This standard specifies the quarantine and identification methods for the phytophthora infestans. This standard applies to plants, plant products, and entrained soils and mediums that target the host of Phytophthora capsici (see Appendix A). Quarantine and identification of the phytophthora root rot pathogen.

2 Phytophthora root rot pathogen basic information

Scientific name. PhytophthoramedicaginisE.M.Hans.etD.P.Maxwel. Taxonomic status. Chromista, Oomycota, Peronosporea, Peronospora- Les), Peronosporaceae, Phytophthora. Transmission route. soil, flowing water, cultivation medium, seedlings, root tissues can all be transmitted and transmitted, and dry grasses traded and transported also have long distances. The risk of transmission. See Appendix A for additional information on P. grisea root rot.

3 Methodology

Pathological symptoms of host plants, morphological characteristics of pathogenic bacteria, and sequence characteristics of pathogenic bacteria genome target fragments collection.

4 Instruments and apparatus

4.1 Instruments Clean bench, light incubator, artificial climate box, biological microscope, electronic balance (sensibility 0.01g), autoclave, blood count , Constant temperature water bath, High-speed refrigerated centrifuge, Ice maker, Spectrophotometer, PCR amplification instrument, Electrophoresis apparatus, Gel imaging system, Fluorescent PCR The instrument. 4.2 Appliances Beaker, flask, graduated cylinder, petri dish, alcohol lamp, tweezers, scissors, scalpel, inoculation needle, slide, coverslip, plastic mortar, pipette Device, pipette tips, centrifuge tubes.

5 Reagents and culture media

5.1 Reagent materials Unless otherwise specified, all reagents were of analytical grade. Pimamarycin (C33H47NO13), Ampicillin Sodium Salt (C16H18N3O4SNa), Rifampicin sodium salt (C43H58N4O12), pentachloronitrobenzene (C6C15NO2), calcium carbonate (CaCO3), sodium hypochlorite (NaOCl). Tris(hydroxymethyl)aminomethane (C4H11NO3), Sodium Chloride (NaCl2), Hydrochloric acid (HCl), Tetrasodium ethylenediaminetetraacetate (C10H4N2O8Na2), ......

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