GB/T 34756-2017 English PDF

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GB/T 34756-2017: Porcine rotavirus disease -- RT-PCR for detecting virus acid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34756-2017	199	Add to Cart	3 days	Porcine rotavirus disease -- RT-PCR for detecting virus acid	Valid

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Basic data

Standard ID: GB/T 34756-2017 (GB/T34756-2017)
Description (Translated English): Porcine rotavirus disease -- RT-PCR for detecting virus acid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,181
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34756-2017: Porcine rotavirus disease -- RT-PCR for detecting virus acid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Porcine rotavirus disease-RT-PCR for detecting virus acid ICS 11.220 B41 National Standards of People's Republic of China Porcine rotavirus virus RT-PCR detection method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAT/TC181) centralized. This standard drafting unit. Northeast Agricultural University, China Animal Health and Epidemiology Center, Henan Animal Husbandry School of Economics. The drafters of this standard. Joe pay ho, Shao Weixing, Wei Rong, Cui Wen, Tang Lijie, Li Yijing, Jiang Yanping, Sun Yingxue, Li Weihua, Xu Yaohui. Porcine rotavirus virus RT-PCR detection method

1 Scope

This standard specifies the reagents, equipment, procedures for the detection of porcine rotavirus RT-PCR. This standard applies to clinical samples of pigs (fresh faeces and small intestine) and cell culture of porcine rotavirus nucleic acid detection.

2 Abbreviations

The following abbreviations apply to this document. AMV. Avianmyoblastosis virus bp. basepair DEPC. Diethypyrocarbonate DNA. Deoxyribonucleic acid dNTPs. deoxyribonucleosidetriphosphates PBS. Phosphate-bufferedsalinebuffer PCR. Polymerase chain reaction (polymerasechainreaction) RNA (ribonucleic acid) RRI. RNase inhibitor (RNaseribonucleaseinhibitor) RT. Reverse transcriptase RT-PCR. Reverse transcription-polymerase chain reaction (reversetranscriptase-polymerasechainreaction) Taq enzyme Taq DNA polymerase (Taq DNA polymerase)

3 reagent

Unless otherwise specified, the reagents used shall be of analytical grade. Reagents for viral RNA extraction should be performed using RNase-free containers Packing. 3.1 chloroform. Store at room temperature. 3.2 isopropanol. 3.3 75% ethanol. 3.4 diethyl pyrophosphate (DEPC). 3.5 Taq enzyme. 3.6 AMV. 3.7 DNA molecular weight standards (DL2000bp). 3.8 electrophoresis buffer (TAE) (see formula A.1). 3.9 1.5% agarose (Formulation see A.3). 3.10 Porcine rotavirus positive samples and negative samples (see Appendix B).

4 equipment

4.1 high-speed refrigerated centrifuge. ......

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