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GB/T 34746-2017 English PDF

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GB/T 34746-2017: Genotyping method of canine parvovirus
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 34746-2017299 Add to Cart 3 days Genotyping method of canine parvovirus Valid

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Basic data

Standard ID: GB/T 34746-2017 (GB/T34746-2017)
Description (Translated English): Genotyping method of canine parvovirus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 15,157
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34746-2017: Genotyping method of canine parvovirus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Genotyping method of canine parvovirus ICS 11.220 B41 National Standards of People's Republic of China Canine parvovirus genotyping methods Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted unit. Yangzhou University, Jiangsu Agricultural Science and Technology Vocational College, China Animal Health and Epidemiology Center, People's Republic of China Exit Inspection and Quarantine of Shenzhen The main drafters of this standard. Liu Wenbo, Zhang Xiaorong, Wang Haiyan, Xu Xiangming, Shao Weixing, Wang Tao, Liu Jing, Zhan Aijun. Canine parvovirus genotyping methods

1 Scope

This standard specifies the canine parvovirus (canine parvovirus, CPV) genotyping method requirements. This standard applies to canine parvovirus pathogen typing, canine parvovirus infection surveillance and epidemiological investigation.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. CPV. Canine parvovirus PCR. Polymerase chain reaction (polymerasechainreaction) DNA. Deoxyribonucleic acid RNA (ribonucleic acid) PBS. Phosphate-bufferedsalinebuffer EDTA. Ethylenediaminetetraacetic acid Taq enzyme Taq DNA polymerase (Taq DNA polymerase) Tris. Tris (hydroxymethyl) aminomethane] dNTPs. deoxyribonucleotide triphosphatesmixture dATP. Deoxyadenosine triphosphate (deoxyadenosine triphosphate) dGTP. Deoxyguanosine triphosphate (deoxyguanosine triphosphate) dCTP. deoxycytidinetriphosphate dTTP. deoxythymidinetriphosphate RNase. Ribonuclease

4 instruments

4.1 PCR amplification instrument. 4.2 high-speed desktop refrigerated centrifuge can be controlled temperature to 4 ℃, centrifuge speed up to 12000r/min above. 4.3 tissue grinder or mortar. 4.4 2 ℃ ~ 8 ℃ and -20 ℃ freezer and ultra-low temperature refrigerator below -70 ℃. 4.5 nucleic acid electrophoresis apparatus and horizontal electrophoresis device. 4.6 Micropipette range 0.2μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 20μL ~.200μL and 100μL ~ 1000 μL pipettes and equipped with tips that match the pipettes.
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