GB/T 32132-2015 English PDF

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GB/T 32132-2015: DNA identification of wool, cashmere, ducks down, gooses down animal materials -- Real-time fluorescence PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 32132-2015	129	Add to Cart	3 days	DNA identification of wool, cashmere, ducks down, gooses down animal materials -- Real-time fluorescence PCR method	Valid

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Basic data

Standard ID: GB/T 32132-2015 (GB/T32132-2015)
Description (Translated English): DNA identification of wool, cashmere, ducks down, gooses down animal materials -- Real-time fluorescence PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40;A49
Classification of International Standard: 07.080
Word Count Estimation: 6,646
Date of Issue: 2015-10-09
Date of Implementation: 2016-11-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Announcement 2015 No.31
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the wool, cashmere, duck, goose animal material DNA components real-time PCR qualitative test. This Standard applies to identify animal materials of wool, cashmere, duck, goose and other animal-derived DNA components.

GB/T 32132-2015: DNA identification of wool, cashmere, ducks down, gooses down animal materials -- Real-time fluorescence PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Animal material (wool, cashmere, duck, goose) property real-time PCR method for DNA identification method) ICS 07.080 A40/49 National Standards of People's Republic of China Animal material (wool, cashmere, duck, goose) genus DNA identification method of real-time PCR method DNAidentificationofwool, cashmere, duck'sdown, goose'sdownanimal Issued on. 2015-10-09 2016-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) and focal points. This standard was drafted. Shenzhen Academy of Metrology and Quality Inspection, China Testing Technology Research Institute, Zhuhai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chen Guopei, Lai Xintian, Lin Lin, Zhou Hua, of Adding Mo Qiuhua, Hong Xiaoming, complex Tang Jun, Hu Kexin, He Yongsheng, Young Friends of the red, LATIN Yangguo Wu, Ze. Animal material (wool, cashmere, duck, goose) genus DNA identification method of real-time PCR method

1 Scope

This standard specifies the wool, cashmere, duck, goose animal material DNA components real-time PCR qualitative test. This standard applies to identify animal materials wool, cashmere, duck, goose and other animal-derived DNA components.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 The standard use of sodium dodecyl sulfate - guanidine isothiocyanate -β- mercaptoethanol or other equivalent method of DNA extraction method, to obtain for real-time PCR for detection of DNA. At the same time according to the species-specific gene fragments of various animals, fluorogenic primers designed to specifically, by real-time Fluorescent PCR reaction, detected the presence of a variety of animal-derived DNA components, so as to achieve animal material properties of DNA into Sub qualitative detection purposes.

4 equipment and appliances

4.1 real-time PCR instrument. 4.2 Clean Benches. 4.3 Centrifuge (highest number of rotations 15000g). 4.4 Micropipettes. 0.2μL ~ 2μL, 1μL ~ 10μL, 2μL ~ 20μL, 20μL ~ 200μL, 200μL ~ 1000μL. 4.5 constant temperature water bath. (65 ± 1) ℃. 4.6 ultrapure water system (resistivity > 18.2MΩ · cm). 4.7 UV spectrophotometer. 230nm, 260nm, 280nm.

5 Reagents and materials

Unless otherwise indicated in the analysis using only analytical reagent grade water should be consistent with GB/T 6682 a water requirements. 5.1 2% SDS solution. Weigh 2gSDS (sodiumdodecylsulfate, sodiumsalt, sodium dodecyl sulfate) powder was added 5mL1mol/LTris-HCl (pH8.0) [Tris-. tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane] And 4mL0.5mol/LEDTA (pH8.0) (ethylenediaminetetraaceticacid, ethylene diamine tetra-acetic acid), add water to 100mL, stored at room temperature. 5.2 guanidine isothiocyanate extract. Weigh 47.25g guanidine isothiocyanate, 1.75g \u200b\u200bsodium chloride, 4gPVPK-40 (Polyvinylpyrrolidone K-40, polyvinyl pyrrolidone K-40), was added 5mL1mol/LTris-HCl (pH8.0) and 4mL0.5mol/LEDTA ......

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