GB/T 31807-2015 English PDFUS$489.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 31807-2015: Detection and identification of Phymatotrichopsis omnivora (Duggar) Hennebert Status: Valid
Basic dataStandard ID: GB/T 31807-2015 (GB/T31807-2015)Description (Translated English): Detection and identification of Phymatotrichopsis omnivora (Duggar) Hennebert Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 23,231 Date of Issue: 2015-07-03 Date of Implementation: 2015-11-27 Regulation (derived from): National Standard Announcement 2015 No.22 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 31807-2015: Detection and identification of Phymatotrichopsis omnivora (Duggar) Hennebert---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Phymatotrichopsis omnivora (Duggar) Hennebert ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of cotton root rot Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Zhuhai People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Huili, Zhang Weidong, the military section of Victoria, Xu Ying, Chen Xianfeng, Cuijun Xia, Du Hongzhong, Shan Wu products. Quarantine and identification of cotton root rot1 ScopeThis standard specifies the method for inspection and identification of cotton root rot fungi. This standard applies to quarantine and identification of cotton root rot host plants plants, seeds and media.2 Equipment and Reagents2.1 Equipment Microscopes, dissecting microscope, the balance (a sense of volume 1/100g, 1/10000g), pH meter, ice machines, water meter, a vortex, a desktop centrifugal Machines, high-speed refrigerated centrifuge, low temperature refrigerators, refrigerator-freezers refrigerator, clean bench, PCR instrument, electrophoresis, horizontal electrophoresis tank gel imager, Micropipette (2μL, 20μL, 100μL, 200μL, 1000μL). 2.2 Reagents Unless otherwise specified, all reagents were analytical grade use. It includes sterile double distilled water, sodium hypochlorite, liquid nitrogen, proteinase K, magnesium hydroxide, Sodium, ammonium acetate, potassium chloride, ethanol, ethidium bromide, chloroform, isopropanol, isoamyl alcohol, TaqDNA polymerase, dNTP mix Material, 10 × PCR buffer, agarose, of DNA molecular weight standards, CTAB, Tris/EDTA/SDS extraction buffer (TES), Tris Borate EDTA buffer (TBE), Tris/EDTA buffer (TE) and the like. Isolation medium, sporulation medium, MA Potato dextrose agar (PDA) preparation methods and procedures in Appendix C. Identify bacteria 3 3.1 Symptom Check The root cause of the roots of plants suspected a dissecting microscope examination, the cable with bacteria or sclerotia pick. Seeds samples, pick and choose entrainment Plant residues, especially sick deformed roots. With wet soil medium sieve to pick out one of the sclerotia (see Appendix B). 3.2 Isolation of pathogens 3.2.1 root cause isolation and culture of bacterium cable In dissecting microscope, the bacteria on the root cause of the cable cover pick, the mycelium was washed with sterile water fragments, washed and placed on a separate medium, 28 ℃ culture dark day observation, to be grow hyphae, hyphae picked tip transferred to PDA plates purification. 3.2.2 Isolation and culture of pathogens on plant debris In tap water plant debris washed, cut at the junction of healthy tissue disease, with 1% sodium hypochlorite for surface disinfection 3min, sterile water 3 times, dry surface moisture after sterilization paper and placed on a separate medium, 28 ℃ dark culture, observe every day, to be grown mycelium, bacteria picked Transfer to a wire tip purification on PDA plates. 3.2.3 Isolation sclerotia on pathogens Isolation and culture of the pathogen sclerotia with 3.2.2. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 31807-2015_English be delivered?Answer: Upon your order, we will start to translate GB/T 31807-2015_English as soon as possible, and keep you informed of the progress. 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