GB/T 31805-2015 English PDFUS$339.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 31805-2015: Detection and identification of Cowpea severe mosaic virus Status: Valid
Basic dataStandard ID: GB/T 31805-2015 (GB/T31805-2015)Description (Translated English): Detection and identification of Cowpea severe mosaic virus Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 16,178 Date of Issue: 2015-07-03 Date of Implementation: 2015-11-27 Regulation (derived from): National Standard Announcement 2015 No.22 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This Standard specifies the cowpea severe mosaic virus quarantine identified serology, molecular biology and detection methods. This Standard applies may carry heavy cowpea mosaic virus in a plant propagation material (seeds, plants) and for quarantine and identification products. GB/T 31805-2015: Detection and identification of Cowpea severe mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Cowpea severe mosaic virus ICS 65.020.01 B16 National Standards of People's Republic of China Cowpea severe mosaic virus quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chen Qing, Li Bin, Fang Zhipeng, Huang Feng, Liao Furong, Chen Yun, Lin Shiming. Cowpea severe mosaic virus quarantine and identification methods1 ScopeThis standard specifies the cowpea severe mosaic virus quarantine identified serology, molecular biology and detection methods. This standard applies may carry heavy cowpea mosaic virus in a plant propagation material (seeds, plants) and for quarantine and identification products.2 Equipment and Reagents2.1 Equipment PCR, quantitative PCR, autoclaves, ice maker, ultra-low temperature freezer, a conventional refrigerator, whirlpool oscillator, electrophoresis, gel imaging system EC, micro-mills, microplate reader, washer, microbalance (sense of volume. 0.001g), horizontal electrophoresis tank, desktop high-speed refrigerated centrifuge, water bath, pH meter, pipettes (1000μL, 200μL, 20μL, 2μL), 96 well microtiter plates, mortar or the like; pest control greenhouse. 2.2 Reagents Unless otherwise specified, all reagents were of analytical grade. PCR buffer, dNTPs (dATP, dTTP, dCTP, dGTP), DNA Polymerase, primers and probes, agar, ELISA detection reagent in Appendix B. Preparation of test sample 3 3.1 Seed Picked deformity seed sown in sterilized soil, grow to be 3 to 4 leaves, the plants will show symptoms of number of plants not showing symptoms Group (10 per group) and number. Collecting leaves divided into two parts, respectively, as required for enzyme-linked assays and molecular testing. 3.2 Plant Symptoms (such as blade malformation, mosaic, mottled, etc.) number of plants individually tested. No symptoms of the grouping and number detection, grouping And detection methods with 3.1. Double antibody sandwich enzyme-linked immune assay.4 Detection and Identification4.1 pairs immunosorbent assay antibody sandwich enzyme-linked Sample preparation was added to the supernatant which had been coated CPSMV antibody ELISA plate, make the DAS-ELISA. Each sample was added to parallel Two holes. Let the negative and positive controls [healthy plant tissue as negative control, infected plant tissue CPSMV to make positive According to which the negative control and the type of material (such as seed or blade) should be consistent with the test sample], sample extraction buffer as control, not With detection reagents or kits according to the instructions. For details, see Appendix B. 4.2 RT-PCR Total RNA was extracted from the samples and controls, after reverse transcription synthesis of cDNA, PCR amplification. Healthy plant tissue as negative for Photos, infection of plant tissue CPSMV as positive control, with ultra-pure water as control. For details, see Appendix C. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 31805-2015_English be delivered?Answer: Upon your order, we will start to translate GB/T 31805-2015_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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