GB/T 31804-2015 English PDF

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GB/T 31804-2015: Detection and identification of Apple scar skin viroid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31804-2015	319	Add to Cart	3 days	Detection and identification of Apple scar skin viroid	Valid

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Basic data

Standard ID: GB/T 31804-2015 (GB/T31804-2015)
Description (Translated English): Detection and identification of Apple scar skin viroid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,152
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31804-2015: Detection and identification of Apple scar skin viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Apple scar skin viroid ICS 65.020.01 B16 National Standards of People's Republic of China Apple rust fruit quarantine and identification of viruses Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Zhuhai Exit Inspection and Quarantine, the People's Republic of China Exit Inspection and Quarantine and the State of Xiamen, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Qingdao Municipal Academy of Agricultural Sciences, People's Republic of China Shandong CIQ. The main drafters. Zhang Yongjiang, Liao force, Liao Furong, Xin Yan Yan, Deng Cong Liang, Shi visit, Mayon group, Li Mingfu, MATURAL Weimei Sheng, Zhang Cheng standard, Wu Xinghai. Apple rust fruit quarantine and identification of viruses

1 Scope

This standard specifies the method for inspection and identification apple rust viroid on host plants and symptoms of genomic features. This standard applies to the detection and identification of possible rust with apple viroid plant material. 2 equipment, appliances, and reagents 2.1 Equipment Electronic analytical balance (0.001g), vertical electrophoresis tank, a small centrifuge, refrigerated centrifuge desktop, ordinary PCR, real-time PCR Instrument, electrophoresis system, pH meter, gel imaging system, 4 ℃ refrigerator, clean benches, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, Vortex, microwave. 2.2 Appliances Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL), pipette tips, centrifuge tubes (0.2mL, 0.5mL, 1.5mL) and RESEARCH Beryllium. 2.3 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. R-PAGE reagent Appendix B; RT-PCR reagents in Appendix C; real-time fluorescent RT-PCR reagents in Appendix D.

3 Detection

3.1 R-PAGE assay R-PAGE assay Appendix B. 3.2 RT-PCR Detection RT-PCR, see Appendix C. 3.3 real-time fluorescent RT-PCR. Real-time fluorescent RT-PCR, see Appendix D.

4 results found

Chapter 3 samples were different in any two principle methods tested positive, the sample can be determined with ASSVd. ......

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