GB/T 31802-2015 English PDF

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GB/T 31802-2015: Detection and identification of Tomato spotted wilt virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31802-2015	319	Add to Cart	3 days	Detection and identification of Tomato spotted wilt virus	Valid

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Basic data

Standard ID: GB/T 31802-2015 (GB/T31802-2015)
Description (Translated English): Detection and identification of Tomato spotted wilt virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,140
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-20
Quoted Standard: SN/T 2122
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the method for identification of quarantine phytosanitary Tomato spotted wilt virus. This Standard applies to quarantine and identification of plant seeds, seedlings and other propagation material of tomato spotted wilt virus.

GB/T 31802-2015: Detection and identification of Tomato spotted wilt virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Tomato spotted wilt virus ICS 65.020.01 B16 National Standards of People's Republic of China Tomato spotted wilt virus quarantine and identification methods Issued on. 2015-07-03 2015-11-20 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. MATURAL, Lu Jie, Ma Jie, hole Jun, Wang Xiufen, Du Zhixin, Zhang Yongjiang, Weimei Sheng. Tomato spotted wilt virus quarantine and identification methods

1 Scope

This standard specifies the method for identification of quarantine phytosanitary Tomato spotted wilt virus. This standard applies to quarantine and identification of plant seeds, seedlings and other propagation material of tomato spotted wilt virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 equipment and reagents

3.1 Equipment Microplate reader, the balance (a sense of volume 1/10000g), pH meter, trace juicer, PCR, real-time PCR instrument, electrophoresis, electrophoresis tank, Gel imaging system, isolate the greenhouse, water bath, cold refrigerator. Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), ELISA plate, mortar tubes, flower pots, sterilization Soil. 3.2 Reagents Enzyme-linked immunosorbent assay reagent see Appendix B, RT-PCR detection reagent in Appendix C, real-time fluorescent RT-PCR detection reagent see Appendix D, biological assay reagent see Appendix E.

4 Sampling and Sample Preparation

4.1 Sampling Sampling conducted in accordance with the provisions of SN/T 2122's. 4.2 Preparation of seed samples Seeds (emphasis picked malformation, immature seed) sown in sterilized soil, grown and symptoms observed at 25 ℃. Grow to be 3 ~ 4 leaves, the plants will show symptoms of number of plants did not show symptoms of the packet (10 as a group) and number. Leaves collected for enzyme-linked immunosorbent Determination, by RT-PCR, real-time fluorescence RT-PCR, and biological assays. 4.3 Preparation of the sample seedlings The seedlings were grown in a greenhouse in isolation, growth and symptoms observed at 25 ℃. Symptomatic seedlings individually tested. No symptoms of sub Group testing, grouping method and detection methods with 4.2. ......

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