GB/T 31801-2015 English PDF

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GB/T 31801-2015: Detection and identification of Phytophthora syringae Kleb.
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31801-2015	319	Add to Cart	3 days	Detection and identification of Phytophthora syringae Kleb.	Valid

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Basic data

Standard ID: GB/T 31801-2015 (GB/T31801-2015)
Description (Translated English): Detection and identification of Phytophthora syringae Kleb.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,190
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31801-2015: Detection and identification of Phytophthora syringae Kleb.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora syringae Kleb. ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of Phytophthora syringae Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, Tianjin, People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Du Hongzhong, Roca Feng, Wu Shan goods, Yan Jin. Quarantine and identification of Phytophthora syringae

1 Scope

This standard specifies the method for inspection and identification of Phytophthora syringae. This standard applies to the host for Phytophthora syringae seedlings, branches, leaves, fruits and entrained soil and medium cloves Phytophthora Identification.

2 apparatus and appliances

2.1 Instrument Biological microscope, clean benches, light incubator, electronic scales (1/1000g), autoclaves, refrigerators, PCR amplification, fluorescence PCR amplification, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath. 2.2 Appliances Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank. 3 reagents, materials and media 3.1 Reagents β- sitosterol, pimaricin (Pimaricin), ampicillin sodium salt (Ampicilin), sodium salt rifampicin (rifampicin,), five clonazepam Benzene (pentachloronitrobenzene), hymexazol (hymexazol), CaCO3, agarose, Tris-HCl, MgCl2, HCl, edetate Acid tetrasodium salt (EDTA), NaOH, NaOAc, KCl, Tris, cetyl trimethyl ammonium bromide (CTAB), TAE, ethanol (etha- nol), chloroform (chloroform), isopropyl alcohol (isopropanol), iso-amyl alcohol (isoamylalcohol), SYBRGreenI nucleic acid dyes, Proteinase K, TaqDNA polymerase, dNTP, universal primers ITS5 and ITS4, real-time PCR primer PS-F and PS-R and Taq- Man-MGB probe PS-Pr. 3.2 Material Agar, V8 juice, summer oak blades or apple or pear, orange skin or in leaves. 3.3 Medium PDA medium, V8 medium, V8 medium orange leather, V8 leaves of medium, selective medium PARPH-V8. Equipment See Appendix A. Matrix Formulation

4 identification of quarantine

4.1 Symptom Check Check the host plant roots, stems, leaves, fruits and other parts, to have withered, fruit rot, root rot, crown rot symptoms and flow of plastic plants, as well as around the root ......

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