GB/T 31795-2015 English PDF

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GB/T 31795-2015: Detection and identification of Tomato black ring virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31795-2015	399	Add to Cart	4 days	Detection and identification of Tomato black ring virus	Valid

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Basic data

Standard ID: GB/T 31795-2015 (GB/T31795-2015)
Description (Translated English): Detection and identification of Tomato black ring virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 19,154
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Quoted Standard: SN/T 1840; SN/T 2122
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the tomato black ring virus DAS-ELISA, quarantine and identification of RT-PCR, MACS RT-PCR, real-time fluorescence RT-PCR, detection and identification of host immune electron microscopy and other methods. This Standard applies to plant material, including vegetative propagation material, quarantine and identification of seeds and seedlings of tomato black ring virus.

GB/T 31795-2015: Detection and identification of Tomato black ring virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Tomato black ring virus ICS 65.020.01 B16 National Standards of People's Republic of China Tomato black ring virus quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yang Cuiyun at Chui, Guo Jingze, Wei Yadong, Deng Cong Liang, Qiao Yanyan, Cuixue Hui, Hu Peilong, Sun Juan. Tomato black ring virus quarantine and identification methods

1 Scope

This standard specifies the tomato black ring virus DAS-ELISA, RT-PCR, MACS RT-PCR, real-time fluorescence RT-PCR, Kam Do not host quarantine detection and identification of immune electron microscopy. This standard applies to plant material, including vegetative propagation material, quarantine and identification of seeds and seedlings of tomato black ring virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy method SN/T 2122 entry and exit quarantine of plants and plant products sampled 3 equipment, facilities, equipment and reagents 3.1 Equipment Microplate reader, PCR, real-time PCR instrument, clean benches, electronic scales (sense of volume 0.001g), electrophoresis, electrophoresis tank, into the gel Imaging systems, transmission electron microscope, water bath, high-speed refrigerated centrifuge, ultra-low temperature freezer (-80 ℃), autoclaves, ice maker, microwave Furnace, a vortex. 3.2 Facilities Plant quarantine nursery. 3.3 Appliances Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL) and the corresponding RNase-free tips, RNase-off Heart tube, PCR tubes, mortar, sample bags, labels, tweezers, magnifying glass, magnetic racks. 3.4 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. TBRV antibodies, beads (superparamagnetic polystyrene beads, the diameter of 280nm, the surface marked with tosyl), Trizol, Diethylpyrocarbonate (DEPC), liquid nitrogen, chloroform, isoamyl alcohol, isopropanol, ethanol, AMV reverse transcriptase, RNA inhibitors, Taq DNA polymerase, DNA molecular markers, ethidium bromide, agarose, bromophenol blue. Serological and molecular biology reagent preparation see Appendix B.

4 symptom observation and sampling

4.1 Observation of Symptoms Spot symptoms of viral infestation of plant material on entry quarantine main observation, TBRV Damage symptoms described in Appendix A. ......

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